glypican-4 Double Nickase Plasmid (h): sc-405200-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- glypican-4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- glypican-4 Double Nickase Plasmid (h) and glypican-4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPC4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: glypican-4 Antibody (AT51E3): sc-517403
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
glypican-4 Double Nickase Plasmid (h) | sc-405200-NIC | 20 µg | $410.00 | |||
glypican-4 Double Nickase Plasmid (h2) | sc-405200-NIC-2 | 20 µg | $410.00 |
Human GPC4 encodes glypican-4, a glycosylphosphatidylinositol (GPI)-anchored heparan sulfate proteoglycan that localizes to the cell surface and modulates extracellular ligand presentation. By binding growth factors, morphogens, and extracellular matrix components, glypican-4 can influence receptor tyrosine kinase signaling and developmental pathways such as Wnt/β-catenin and Hedgehog, shaping cell proliferation, differentiation, and tissue patterning. GPC4 activity has been studied in contexts including neurodevelopment, synapse-related processes, and metabolic regulation through adipose and insulin-responsive signaling networks. Altered expression or regulation of GPC4 has been reported across multiple disease-relevant research areas, supporting its use as a target to interrogate dysregulated signaling and cell–cell communication.
glypican-4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPC4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPC4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPC4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPC4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.