GAK Antibody (G-10): sc-137066
- GAK Antibody (G-10) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-360 of GAK of rat origin
- recommended for detection of GAK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG2b BP-HRP is the preferred secondary detection reagent for GAK Antibody (G-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with GAK Antibody (G-10) (see ordering information below).
QUICK LINKS
SEE ALSO...
GAK Antibody (G-10) is a mouse monoclonal IgG2b antibody that detects GAK in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GAK, or cyclin G-associated kinase, plays a pivotal role in cell cycle regulation by interacting with cyclin G, which is crucial for proper cell cycle progression. GAK is primarily located in the cytoplasm and is involved in phosphorylating various substrates, influencing cellular processes such as growth and division. Notably, anti-GAK antibody (G-10) has shown GAK co-immunoprecipitates with cyclin G and Cdk5, highlighting GAK′s importance in regulating cyclin-dependent kinases. The interaction between GAK and cyclin G suggests a mechanism for modulating cyclin-dependent kinase activity, thereby impacting cell cycle transitions. Cyclin G expression is induced shortly after growth stimulation, indicating a role in responding to cellular signals, and continuous expression without clear cell cycle dependency suggests functions beyond cell cycle regulation. GAK monoclonal antibody (G-10) serves as an essential tool for studying cell cycle control dynamics and molecular interactions governing cellular proliferation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GAK Antibody (G-10) References:
- GAK, a regulator of clathrin-mediated membrane trafficking, localizes not only in the cytoplasm but also in the nucleus. | Sato, J., et al. 2009. Genes Cells. 14: 627-41. PMID: 19371378
- GAK, a regulator of clathrin-mediated membrane traffic, also controls centrosome integrity and chromosome congression. | Shimizu, H., et al. 2009. J Cell Sci. 122: 3145-52. PMID: 19654208
- The clathrin-binding and J-domains of GAK support the uncoating and chaperoning of clathrin by Hsc70 in the brain. | Park, BC., et al. 2015. J Cell Sci. 128: 3811-21. PMID: 26345367
- GAK is phosphorylated by c-Src and translocated from the centrosome to chromatin at the end of telophase. | Fukushima, K., et al. 2017. Cell Cycle. 16: 415-427. PMID: 28135906
- LncRNA OIP5-AS1/cyrano suppresses GAK expression to control mitosis. | Kim, J., et al. 2017. Oncotarget. 8: 49409-49420. PMID: 28472763
- Chemical genetic identification of GAK substrates reveals its role in regulating Na+/K+-ATPase. | Lin, AW., et al. 2018. Life Sci Alliance. 1: e201800118. PMID: 30623173
- SGC-GAK-1: A Chemical Probe for Cyclin G Associated Kinase (GAK). | Asquith, CRM., et al. 2019. J Med Chem. 62: 2830-2836. PMID: 30768268
- Dynamics of Auxilin 1 and GAK in clathrin-mediated traffic. | He, K., et al. 2020. J Cell Biol. 219: PMID: 31962345
- Functional genomic landscape of cancer-intrinsic evasion of killing by T cells. | Lawson, KA., et al. 2020. Nature. 586: 120-126. PMID: 32968282
- The cyclin G-associated kinase (GAK) inhibitor SGC-GAK-1 inhibits neurite outgrowth and synapse formation. | Egawa, J., et al. 2022. Mol Brain. 15: 68. PMID: 35883152
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAK Antibody (G-10) | sc-137066 | 200 µg/ml | $322.00 | |||
GAK Antibody (G-10): m-IgG2b BP-HRP Bundle | sc-548502 | 200 µg Ab; 10 µg BP | $361.00 |