Fibrillarin Anticuerpo (G-8) recomendado para detectarFibrillarin de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

Fibrillarin Anticuerpo (G-8): sc-374022

Fibrillarin Anticuerpo (G-8) clasificado 4.7 de 5 por 7.
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Ficha técnica

    • Anti-Fibrillarin Anticuerpo G-8 es un monoclonal de ratón IgG1 κ Fibrillarin Anticuerpo, ver las 8 publicaciones, proporcionado como 200 µg/ml
    • específico para un epítopo localizado entre los amino ácidos 99-127 de la región interna de Fibrillarin de origen human
    • Fibrillarin Anticuerpo (G-8) es recomendado para detectar Fibrillarin de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA; también reactivo con otras especies, incluyendo anyd canine, bovine and porcine
    • Fibrillarin Anticuerpo (G-8) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Fibrillarin (G-8): sc-374022.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Fibrillarin Antibody (G-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with Fibrillarin Antibody (G-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I was wondering whether you have any information regarding room temp incubation of this antibody (sc-374022) for IF on cells? I'm planning to use this for a drug screen and the system is not amenable to o/n incubation at 4C. Thanks.

Preguntado por: Ceryl
Thank you for your question. Yes, we do recommend incubating with this antibody for 60 minutes at room temperature. Please refer to our immunofluorescence protocol for more detailed information.
Respondida por: Technical Support
Fecha de publicación: 2019-03-03

What application is the blocking peptide sc-374022 P appropriate for?

Preguntado por: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Respondida por: Technical Support
Fecha de publicación: 2017-02-15
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Rated 3 de 5 por de Works ok for IF. akI got a sample of this antibody. Clear but weak IF with 1:50 dilution.
Fecha de publicación: 2017-07-27
Rated 5 de 5 por de great features! Works well for immunofluorescence and Wester blotting in human HT1080 cancer cells and MEF cells.
Fecha de publicación: 2017-07-10
Rated 5 de 5 por de an excellent antibody for both WB and IF This antibody works very well for WB (40 ug U251 Lysate) and IF (formaldehyde-fixed HeLa cells) at 1:500 dilution.
Fecha de publicación: 2017-07-05
Rated 5 de 5 por de Great for IF and co-localization experiments I used a sample of this antibody for co-localization studies in mouse ES cells. The nucleolar staining was very specific and significant. generally pleased with outcome. Will recommend it!
Fecha de publicación: 2017-06-05
Rated 5 de 5 por de Broad reactivity. This antibody is capable of detecting Fibrillarin multiple different species. Both staining and blotting assays provide clear and strong signal. This antibody has been used for publishing.
Fecha de publicación: 2017-01-27
Rated 5 de 5 por de Strong Strong, clear bands present in Western blot analysis of Fibrillarin expression in HeLa and NIH/3T3 whole cell lysates. -SCBT QC
Fecha de publicación: 2015-04-18
Rated 5 de 5 por de Superb immunoperoxidase nucleolar and nuclear Superb immunoperoxidase nucleolar and nuclear staining in formalin fixed, paraffin-embedded human breast tissue. -SCBT QC
Fecha de publicación: 2015-04-10
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