FAK Antibody (H-1): sc-1688

FAK Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects FAK p125 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). FAK (H-1) antibody is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. Focal adhesion kinase (FAK) plays a crucial role in cellular signaling and is primarily located at focal adhesions, which are specialized structures that mediate the attachment of cells to the extracellular matrix. This localization is vital as FAK integrates signals from the extracellular environment, influencing cell migration, proliferation, and survival. FAK is concentrated at the basal edge of migrating keratinocytes during wound healing, suggesting significant involvement in the reepithelialization process. FAK′s protein tyrosine kinase activity is upregulated in response to mitogenic stimuli, highlighting importance in cellular responses to growth factors and neurotransmitters. FAK p125′s unique structural organization, as a cytoplasmic protein tyrosine kinase, distinguishes FAK from other kinases, underscoring specialized functions in cellular dynamics and tissue repair.

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FAK Antibody (H-1) References:

1. Activation of transforming G protein-coupled receptors induces rapid tyrosine phosphorylation of cellular proteins, including p125FAK and the p130 v-src substrate.  |  Gutkind, JS. and Robbins, KC. 1992. Biochem Biophys Res Commun. 188: 155-61. PMID: 1329743
2. Regulation of focal adhesion-associated protein tyrosine kinase by both cellular adhesion and oncogenic transformation.  |  Guan, JL. and Shalloway, D. 1992. Nature. 358: 690-2. PMID: 1379699
3. Bombesin, vasopressin, and endothelin stimulation of tyrosine phosphorylation in Swiss 3T3 cells. Identification of a novel tyrosine kinase as a major substrate.  |  Zachary, I., et al. 1992. J Biol Chem. 267: 19031-4. PMID: 1382065
4. Integrin-dependent phosphorylation and activation of the protein tyrosine kinase pp125FAK in platelets.  |  Lipfert, L., et al. 1992. J Cell Biol. 119: 905-12. PMID: 1385445
5. Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin.  |  Hanks, SK., et al. 1992. Proc Natl Acad Sci U S A. 89: 8487-91. PMID: 1528852
6. pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.  |  Schaller, MD., et al. 1992. Proc Natl Acad Sci U S A. 89: 5192-6. PMID: 1594631
7. Autophosphorylation of the focal adhesion kinase, pp125FAK, directs SH2-dependent binding of pp60src.  |  Schaller, MD., et al. 1994. Mol Cell Biol. 14: 1680-8. PMID: 7509446
8. Potential role for focal adhesion kinase in migrating and proliferating keratinocytes near epidermal wounds and in culture.  |  Gates, RE., et al. 1994. Cell Growth Differ. 5: 891-9. PMID: 7986754

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FAK Antibody (H-1) has 111 citations in a variety of scientific publications.

Primary antibodies like Anti-FAK Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FAK Antibody (H-1) Specifications

Alternate Product Name: FAK

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 903-1052

Epitope Target: FAK

Epitope Species: mouse

Product Reactivity/Detection: FAK p125

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 111 citations in various scientific publications

Available Conjugates/Forms for FAK Antibody (H-1): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, PE: Phycoerythrin

The preferred p-FAK antibody is p-FAK Antibody (2D11): sc-81493.