EXOSC10 Double Nickase Plasmid (h): sc-404272-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EXOSC10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EXOSC10 Double Nickase Plasmid (h) and EXOSC10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EXOSC10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EXOSC10 Antibody (B-8): sc-374595
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EXOSC10 Double Nickase Plasmid (h) | sc-404272-NIC | 20 µg | $410.00 | |||
EXOSC10 Double Nickase Plasmid (h2) | sc-404272-NIC-2 | 20 µg | $410.00 |
EXOSC10 encodes an evolutionarily conserved catalytic subunit of the RNA exosome, functioning as a 3′–5′ exoribonuclease that contributes to RNA surveillance and processing in both nuclear and cytoplasmic compartments. It participates in maturation and turnover of rRNA, snoRNA, snRNA, and mRNA, and helps maintain transcriptome integrity by degrading aberrant or improperly processed RNAs. Through these roles, EXOSC10 influences ribosome biogenesis, nucleolar homeostasis, and RNA quality control pathways that intersect with cell-cycle progression and cellular stress responses. Dysregulation of RNA exosome components, including EXOSC10, has been linked to altered RNA metabolism observed across multiple disease contexts, making it a relevant target for mechanistic studies of RNA processing defects.
EXOSC10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EXOSC10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EXOSC10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EXOSC10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EXOSC10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.