Epac Anticuerpo (A-5) recomendado para detectarEpac de mouse, rat, human y hamster origen, mediante WB, IP, IF, IHC(P) y ELISA

Epac Anticuerpo (A-5): sc-28366

Epac Anticuerpo (A-5) clasificado 4.4 de 5 por 10.
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Ficha técnica

    • Anti-Epac Anticuerpo A-5 es un monoclonal de ratón IgG2a (kappa light chain) Epac Anticuerpo proporcionado como 200 µg/ml
    • producido contra los amino ácidos 1-70 de Epac de origen human
    • Epac Anticuerpo (A-5) es recomendado para detectar Epac de mouse, rat, human y hamster origen, mediante WB, IP, IF, IHC(P) y ELISA
    • Epac Anticuerpo (A-5) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Ver m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nuestras altamente recomendadas alternativa a los anticuerpos secundarios convencionales, anti-IgG de ratón.
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Epac (A-5): sc-28366.

Can Epac (A-5): sc-28366 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Preguntado por: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Epac (A-5): sc-28366 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Respondida por: Technical Support
Fecha de publicación: 2017-02-24

Does this recognize epac1 or epac2 or both?

Preguntado por: Marina1
Thank you for your question. This mouse monoclonal antibody, Epac (A-5): sc-28366, is raised against the gene product of RAPGEF3, also designated Epac or Epac1. The Uniprot accession number for the full length protein is O95398. You can find a description with links to NCBI for the gene and protein information on our product page, under the tab "Gene Info" next to "Description" about half way down the page (below the ordering information). https://www.scbt.com/scbt/product/epac-antibody-a-5
Respondida por: Technical Support
Fecha de publicación: 2016-11-21
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Rated 4 de 5 por de Works well in bovine cell lysates I tested this antibody on bovine cell lysates and it works well. There are some smaller bands that show up maybe do to protein degradation.
Fecha de publicación: 2017-10-04
Rated 5 de 5 por de Great antibody It really worked nicely, shown nice bands at 100 kDa at 1:500 dilution.
Fecha de publicación: 2017-04-09
Rated 5 de 5 por de Beautiful results This antibody works well in standard lab applications and will provide great results in samples from several species. It has been published with several times.
Fecha de publicación: 2017-01-27
Rated 5 de 5 por de Worked better than sc Worked better than sc-25632 for WB using 293 cells that over-express protein.
Fecha de publicación: 2015-09-24
Rated 4 de 5 por de This monoclonal was better This monoclonal was better, detecting Epac, although its strongest signal is not Epac.
Fecha de publicación: 2015-07-13
Rated 4 de 5 por de Strong band in WB in Caki and CHO whole cell Strong band in WB in Caki and CHO whole cell lysates. -SCBT QC -SCBT QC
Fecha de publicación: 2015-06-30
Rated 4 de 5 por de Good publishable data in IHC and WB in human Good publishable data in IHC and WB in human TR4 cells. -SCBT Publication Review
Fecha de publicación: 2015-05-30
Rated 5 de 5 por de Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human salivary gland tissue. -SCBT QC
Fecha de publicación: 2015-05-11
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