eIF2C2 Antibody (4F9): sc-53521

eIF2C2 Antibody (4F9) is a mouse monoclonal IgA kappa light chain antibody that detects eIF2C2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-eIF2C2 antibody (4F9) is available as the non-conjugated format. Eukaryotic translation initiation factor 2C (eIF2C) proteins, part of the argonaute family, play a crucial role in RNA interference (RNAi) as integral components of the RNA-inducible silencing complex (RISC) and microRNA (miRNA)-containing ribonucleoprotein particles (miRNPs). eIF2C2 protein, also known as Slicer or argonaute 2, localizes in cytoplasmic processing bodies (P-bodies), where eIF2C2 catalyzes mRNA cleavage. This function is vital for the regulation of gene expression, allowing for the degradation of target mRNAs, thereby influencing various cellular processes such as development, differentiation, and response to stress. The interaction of eIF2C2 with small RNAs, including small interfering RNAs (siRNAs) and miRNAs, facilitates the silencing of target genes, which is essential for maintaining cellular homeostasis and regulating gene expression in response to environmental cues. eIF2C2 works in concert with other eIF2C proteins, such as eIF2C1 (argonaute 1) and Dicer1, to ensure effective gene silencing through a coordinated mechanism.

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eIF2C2 Antibody (4F9) References:

1. Single-stranded antisense siRNAs guide target RNA cleavage in RNAi.  |  Martinez, J., et al. 2002. Cell. 110: 563-74. PMID: 12230974
2. The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis.  |  Carmell, MA., et al. 2002. Genes Dev. 16: 2733-42. PMID: 12414724
3. Structure and conserved RNA binding of the PAZ domain.  |  Yan, KS., et al. 2003. Nature. 426: 468-74. PMID: 14615802
4. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.  |  Meister, G., et al. 2004. Mol Cell. 15: 185-97. PMID: 15260970
5. Argonaute2 is the catalytic engine of mammalian RNAi.  |  Liu, J., et al. 2004. Science. 305: 1437-41. PMID: 15284456
6. Molecular biology. Argonaute journeys into the heart of RISC.  |  Sontheimer, EJ. and Carthew, RW. 2004. Science. 305: 1409-10. PMID: 15353786
7. Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies.  |  Sen, GL. and Blau, HM. 2005. Nat Cell Biol. 7: 633-6. PMID: 15908945
8. Detection of the argonaute protein Ago2 and microRNAs in the RNA induced silencing complex (RISC) using a monoclonal antibody.  |  Ikeda, K., et al. 2006. J Immunol Methods. 317: 38-44. PMID: 17054975
9. The role of GW/P-bodies in RNA processing and silencing.  |  Jakymiw, A., et al. 2007. J Cell Sci. 120: 1317-23. PMID: 17401112
10. Germline AGO2 mutations impair RNA interference and human neurological development.  |  Lessel, D., et al. 2020. Nat Commun. 11: 5797. PMID: 33199684
11. YTHDF1 promotes mRNA degradation via YTHDF1-AGO2 interaction and phase separation.  |  Li, J., et al. 2022. Cell Prolif. 55: e13157. PMID: 34821414
12. AGO2 Protects Against Diabetic Cardiomyopathy by Activating Mitochondrial Gene Translation.  |  Zhan, J., et al. 2024. Circulation. 149: 1102-1120. PMID: 38126189

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF2C2 Antibody (4F9) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF2C2 Antibody (4F9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF2C2 Antibody (4F9) Specifications

Alternate Product Name: eIF2C2

Product Clone Name: 4F9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgA kappa light chain

Amino Acids:

Epitope Target: eIF2C2

Epitope Species: human

Product Reactivity/Detection: eIF2C2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgA kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for eIF2C2 Antibody (4F9): N/A

The preferred eIF2C antibody is eIF2C Antibody (B-3): sc-376696.