DHFR Antibody (A-4): sc-74593
- DHFR Antibody (A-4) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 1-187 representing full length DHFR of human origin
- recommended for detection of DHFR of mouse, rat and human origin by WB, IP, IF and ELISA
- See DHFR (A-9): sc-377091 for DHFR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DHFR Antibody (A-4) for WB applications. These reagents are now offered in bundles with DHFR Antibody (A-4) (see ordering information below).
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SEE ALSO...
DHFR Antibody (A-4) is a mouse monoclonal IgG2a antibody that detects dihydrofolate reductase (DHFR) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DHFR plays a vital role in cellular metabolism by catalyzing the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, which is essential for the synthesis of purines, pyrimidines, and certain amino acids. This enzymatic activity is crucial for DNA synthesis; thus, inhibition of DHFR can lead to cell cycle arrest and apoptosis, making DHFR a significant target in cancer therapy. Notably, the expression of methotrexate-resistant variants of DHFR in normal hematopoietic cells presents a promising strategy to enhance the efficacy of methotrexate treatment by reducing toxicity to healthy tissues. Anti-DHFR antibody (A-4) enables researchers to gain insights into the regulation and function of this important enzyme, facilitating advancements in therapeutic approaches for various diseases.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DHFR Antibody (A-4) | sc-74593 | 200 µg/ml | $322.00 | |||
DHFR Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-534448 | 200 µg Ab; 40 µg BP | $361.00 | |||
DHFR Antibody (A-4): m-IgG2a BP-HRP Bundle | sc-546190 | 200 µg Ab; 10 µg BP | $361.00 |