Cytokeratin 15 Anticuerpo (LHK15) recomendado para detectarCytokeratin 15 de mouse, rat y human origen, mediante WB, IP, IF y IHC(P)

Cytokeratin 15 Anticuerpo (LHK15): sc-47697

Cytokeratin 15 Anticuerpo (LHK15) clasificado 4.6 de 5 por 7.
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Ficha técnica

    • Anti-Cytokeratin 15 Anticuerpo LHK15 es un monoclonal de ratón IgG2a κ Cytokeratin 15 Anticuerpo, ver las 34 publicaciones, proporcionado como 200 µg/ml
    • raised against the last 17 amino acids of Cytokeratin 15 of human origin
    • Cytokeratin 15 Anticuerpo (LHK15) es recomendado para detectar Cytokeratin 15 de mouse, rat y human origen, mediante WB, IP, IF y IHC(P)
    • Cytokeratin 15 Anticuerpo (LHK15) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Cytokeratin 15 (LHK15): sc-47697.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for Cytokeratin 15 Antibody (LHK15) for WB and IHC(P) applications. This reagent is now offered in a bundle with Cytokeratin 15 Antibody (LHK15) (see ordering information below).

Pricing up to buy this:  200ug/ml,  but what volume?

Preguntado por: Shelly99
Thank you for your question. This is provided as 200 ug in 1 ml volume.
Respondida por: Technical Support
Fecha de publicación: 2020-03-25

Dear SAnta cruz,I wanted to try cytokeratin 15 antibody. But since its mouse antibody i am not sure if it will give me lot of background with my mouse skin sample.Is their any possibility to get free small aliquote for trail Please

Preguntado por: zumer
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Respondida por: SCBT Heidelberg Support
Fecha de publicación: 2018-12-14

Can Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Preguntado por: jenni
Thank you for your question. Yes, Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support 8
Fecha de publicación: 2017-01-10
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Rated 5 de 5 por de works very well in histology alone or in coIF Serial sections from human scalp biopsies and mouse ears embedded in OCT or human scalp biopsies embedded in formalin‐fixed paraffin were processed for immunofluorescence as follow: Frozen sections were fixed with neutral buffered 4% paraformaldehyde (PFA) for 15 min at room temperature (RT) and washed three times with phosphate‐buffered saline (PBS). Paraffin sections were processed for their deparaffinization and antigen retrieval. Primary antibody specific for Keratin 15 at 1:400 dilution was incubated overnight (O/N) at 4°C. Sections were then washed three times with PBS and incubated with secondary antibody conjugated to the appropriate fluorophore(s) (for example, Alexa Fluor 647 dilution 1:200) for 1 h at RT. Sections were then washed three times with PBS, counterstained with DAPI, mounted with IF-grate antifade mounting medium and analyzed by fluorescence or confocal microscopy.
Fecha de publicación: 2019-12-04
Rated 4 de 5 por de good antibody the antibody was tested for flow cytometry in mammary cells. worked well.
Fecha de publicación: 2017-11-23
Rated 5 de 5 por de Outstanding IHC staining Great product for IHC staining applications; also nice WB
Fecha de publicación: 2017-01-14
Rated 5 de 5 por de Great IHC(P) data The Cytokeratin 15 Antibody (LHK15): sc-47697 works very well for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has been cited over 10 times for further verification.
Fecha de publicación: 2017-01-10
Rated 5 de 5 por de Excellent images obtained in IF with human Excellent images obtained in IF with human placenta -SCBT Publication Review
Fecha de publicación: 2015-03-07
Rated 5 de 5 por de Works well in IHC with paraffin Works well in IHC with paraffin-embedded human fetal corneal cells -SCBT Publication Review
Fecha de publicación: 2015-03-06
Rated 3 de 5 por de Good single band with some background Good single band with some background -SCBT QC
Fecha de publicación: 2015-02-15
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