cyclin E Antibody (E-4): sc-377100
- cyclin E Antibody (E-4) is a mouse monoclonal IgG2b κ cyclin E antibody, cited in 129 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 367-396 at the C-terminus of cyclin E of rat origin
- cyclin E Antibody (E-4) is recommended for detection of cyclin E1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-cyclin E Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cyclin E Antibody (E-4) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin E Antibody (E-4) (see ordering information below).
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Cyclin E Antibody (E-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects cyclin E protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-cyclin E antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cyclin E plays a pivotal role in regulating cell cycle, particularly during transition from G1 phase to S phase, where cyclin E is essential for cells progressing into DNA synthesis. Cyclin E is primarily located in nucleus, where cyclin E forms a complex with transcription factor E2F, crucial for expressing genes necessary for S phase entry. Cyclin E/E2F complex is temporally regulated, with levels peaking at G1/S boundary, ensuring cells only proceed to DNA replication when adequately prepared. Proper functioning of cyclin E is vital for normal cell division, and dysregulation of cyclin E has been implicated in various cancers, highlighting cyclin E′s importance as a potential therapeutic target.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cyclin E Antibody (E-4) References:
- Cyclin D and cyclin E expression in normal and adenomatous pituitary. | Jordan, S., et al. 2000. Eur J Endocrinol. 143: R1-6. PMID: 10870044
- Cyclin E ablation in the mouse. | Geng, Y., et al. 2003. Cell. 114: 431-43. PMID: 12941272
- Cyclin E as molecular marker in the management of breast cancer: a review. | Lopez-Beltran, A., et al. 2006. Anal Quant Cytol Histol. 28: 111-4. PMID: 16637514
- Deregulation of cyclin E meets dysfunction in p53: closing the escape hatch on breast cancer. | Barton, MC., et al. 2006. J Cell Physiol. 209: 686-94. PMID: 17001684
- Cyclin E deregulation impairs mitotic progression through premature activation of Cdc25C. | Bagheri-Yarmand, R., et al. 2010. Cancer Res. 70: 5085-95. PMID: 20530684
- Cyclin E transgenic mice: discovery tools for lung cancer biology, therapy, and prevention. | Freemantle, SJ. and Dmitrovsky, E. 2010. Cancer Prev Res (Phila). 3: 1513-8. PMID: 21149327
- Cyclin E amplification/overexpression is a mechanism of trastuzumab resistance in HER2+ breast cancer patients. | Scaltriti, M., et al. 2011. Proc Natl Acad Sci U S A. 108: 3761-6. PMID: 21321214
- Cytoplasmic Cyclin E Mediates Resistance to Aromatase Inhibitors in Breast Cancer. | Doostan, I., et al. 2017. Clin Cancer Res. 23: 7288-7300. PMID: 28947566
- USP27-mediated Cyclin E stabilization drives cell cycle progression and hepatocellular tumorigenesis. | Dong, L., et al. 2018. Oncogene. 37: 2702-2713. PMID: 29497124
- Cytoplasmic Cyclin E Expression Predicts for Response to Neoadjuvant Chemotherapy in Breast Cancer. | Karakas, C., et al. 2021. Ann Surg. 274: e150-e159. PMID: 31436549
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin E Antibody (E-4) | sc-377100 | 200 µg/ml | $322.00 | |||
cyclin E Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-529974 | 200 µg Ab; 10 µg BP | $361.00 | |||
cyclin E Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-523188 | 200 µg Ab, 40 µg BP | $361.00 | |||
cyclin E Antibody (E-4): m-IgG2b BP-HRP Bundle | sc-550153 | 200 µg Ab; 10 µg BP | $361.00 | |||
cyclin E Antibody (E-4) AC | sc-377100 AC | 500 µg/ml, 25% agarose | $424.00 | |||
cyclin E Antibody (E-4) HRP | sc-377100 HRP | 200 µg/ml | $322.00 | |||
cyclin E Antibody (E-4) FITC | sc-377100 FITC | 200 µg/ml | $336.00 | |||
cyclin E Antibody (E-4) PE | sc-377100 PE | 200 µg/ml | $349.00 | |||
cyclin E Antibody (E-4) Alexa Fluor® 488 | sc-377100 AF488 | 200 µg/ml | $364.00 | |||
cyclin E Antibody (E-4) Alexa Fluor® 546 | sc-377100 AF546 | 200 µg/ml | $364.00 | |||
cyclin E Antibody (E-4) Alexa Fluor® 594 | sc-377100 AF594 | 200 µg/ml | $364.00 | |||
cyclin E Antibody (E-4) Alexa Fluor® 647 | sc-377100 AF647 | 200 µg/ml | $364.00 | |||
cyclin E Antibody (E-4) Alexa Fluor® 680 | sc-377100 AF680 | 200 µg/ml | $364.00 | |||
cyclin E Antibody (E-4) Alexa Fluor® 790 | sc-377100 AF790 | 200 µg/ml | $364.00 | |||
cyclin E (E-4) Neutralizing Peptide | sc-377100 P | 100 µg/0.5 ml | $69.00 |