Anti-cyclin D1 Anticuerpo A-12 es un monoclonal de ratón IgG2b κ cyclin D1 Anticuerpo, ver las 543 publicaciones, proporcionado como 200 µg/ml
producido contra los amino ácidos 1-295 representando la proteína completa cyclin D1 de origen human
cyclin D1 Anticuerpo (A-12) es recomendado para detectar cyclin D1 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P), FCM y ELISA; puede tener reactividad cruzada con cyclin D2; no tiene reactividad cruzada con cyclin D3
cyclin D1 Anticuerpo (A-12) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
disponible conjugado tanto a TRITC como a Alexa Fluor® 405 para IF, IHC(P) y FCM
Contacte con nosotros para recibir GRATIS 10 µg de muestra de cyclin D1 (A-12): sc-8396.
cyclin D1 para análisis funcionales de las repuestas celulares a Cyclin
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
cyclin D1 Antibody (A-12) is a high quality monoclonal cyclin D1 antibody (also designated CCND1 antibody) suitable for the detection of the cyclin D1 protein of mouse, rat and human origin. cyclin D1 Antibody (A-12) is available as both the non-conjugated anti-cyclin D1 antibody form, as well as multiple conjugated forms of anti-cyclin D1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl-1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
I am looking for a CDK1 antibody suitable to detect a C-terminal GFP CDK1 fusion protein on WB, where CDK1 is truncated resulting in a loss of the last 5 amino acids of CDK1. Which antibody would you recommend? Thanks!
Preguntado por: Sebastian Scheich
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Respondida por: Tech Service 1
Fecha de publicación: 2021-02-04
What is the recommended secondary detection reagent for cyclin D1 Antibody (A-12) in Western blot applications?
Preguntado por: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here:
Rated 4 de
Works for WBIt works good for detecting rat liver protein by western blot
Fecha de publicación: 2021-01-18
Rated 5 de
Fecha de publicación: 2021-01-05
Rated 3 de
Decent Western detection in rat INS-1 cells60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a strong band of ~38 kD and several non-specific bands. Recommend using sc-20044 instead. The attached picture is a triplicate set (identical lanes)
Fecha de publicación: 2019-05-22
Rated 2 de
MDee Ng de
Not really work for IFI used 1:20 dilution for IF staining of chondrocytes on cryo sections, but it does not really work. The one that I used is conjugated to Alexa 647.
Fecha de publicación: 2019-05-06
Rated 4 de
Working for WBIt works for dettecting mouse and human protein by western blot
Fecha de publicación: 2019-01-17
Rated 3 de
Cyclin D1 western blotWe use Cyclin D1 antibody to study proliferation and cell cycle of human amniotic stem cells during their hepatic differentiation. It is an excellent antibody for western blot in human samples. Nevertheless, we have to say that sometimes the weight of the protein does not match the weight of the band.
Fecha de publicación: 2018-05-17
Rated 5 de
UPENNGα 12 Antibody is very good. The band is very stronger.
Fecha de publicación: 2018-04-19
Rated 5 de
Worked Great!!Worked great for human and mouse tissue samples when probing at 1:200 via WB. Highly recommend!
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn