COL6A3 Double Nickase Plasmid (h): sc-402899-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL6A3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COL6A3 Double Nickase Plasmid (h) and COL6A3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COL6A3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COL6A3 Antibody (A-5): sc-515335
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL6A3 Double Nickase Plasmid (h) | sc-402899-NIC | 20 µg | $410.00 | |||
COL6A3 Double Nickase Plasmid (h2) | sc-402899-NIC-2 | 20 µg | $410.00 |
Human COL6A3 encodes the α3 chain of type VI collagen, a key extracellular matrix component that assembles into microfibrillar networks supporting tissue tensile strength and mechanotransduction. COL6A3 contributes to cell–matrix adhesion, matrix organization, and crosstalk with integrin- and TGF-β–linked signaling pathways that influence fibroblast activation, myogenesis, and stromal remodeling. Altered COL6A3 expression or matrix deposition is associated with connective tissue and neuromuscular phenotypes, and it is frequently studied in fibrosis and tumor microenvironment biology where extracellular matrix composition shapes cell migration and differentiation. As a matrix gene with strong context dependence, COL6A3 is commonly interrogated in 3D culture, organoid, and co-culture systems to dissect extracellular matrix–driven changes in cell behavior.
COL6A3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COL6A3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COL6A3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COL6A3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COL6A3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.