Date published: 2025-9-7

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CD81 Anticuerpo (5A6): sc-23962

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Fichas Técnicas
  • CD81 Anticuerpo (5A6) es un monoclonal de ratón IgG1 κ, ver las 127 publicaciones, proporcionado como 200 µg/ml
  • criado contra células OCI-LY8
  • CD81 Anticuerpo (5A6) es recomendado para detectar CD81 de mouse, rat y human origen, mediante WB, IP, IF y FCM
  • CD81 Anticuerpo (5A6) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
  • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
  • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
  • Contacte con nosotros para recibir GRATIS 10 µg de muestra de CD81 (5A6): sc-23962.
  • m-IgG Fc BP-HRP y 1 BP-HRP">m-IgG1 BP-HRP son los reactivos de detección secundarios preferidos para CD81 Anticuerpo (5A6) for WB applications. Estos reactivos se ofrecen ahora en paquetes con CD81 Anticuerpo (5A6)(véase la información de pedido más abajo).

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VER TAMBIÉN ....

El anticuerpo CD81 (5A6) es un anticuerpo monoclonal de ratón IgG1 de cadena ligera kappa diseñado específicamente para la proteína CD81, también conocida como TAPA-1, un destacado miembro de la familia de las tetraspaninas caracterizado por sus cuatro dominios transmembrana. La CD81 se expresa ampliamente en células de origen hematopoyético, neuroectodérmico y mesenquimal, donde desempeña un papel vital en el crecimiento celular, la transducción de señales y la organización de microdominios de membrana. La CD81 sufre modificaciones postraduccionales esenciales, como la palmitoilación y la glucosilación, que son cruciales para su correcta localización, estabilidad e interacciones con otras proteínas de membrana como la CD37, la CD53, la CD19, la CD21 y los antígenos MHC de clase II. El anticuerpo anti-CD81 (5A6) se desarrolló utilizando la línea celular de linfoma B OCI-LY8, asegurando la detección eficaz de CD81 en especies de ratón, rata y humano a través de aplicaciones como western blotting (WB), inmunoprecipitación (IP), inmunofluorescencia (IF) y citometría de flujo (FCM). Disponible tanto en formas conjugadas, que incluyen peroxidasa de rábano picante (HRP), ficoeritrina (PE), isotiocianato de fluoresceína (FITC) y diversos conjugados Alexa Fluor®, como en formas no conjugadas, el anticuerpo monoclonal CD81 (5A6) ofrece opciones versátiles para diversas necesidades experimentales. Al dirigirse a CD81, los investigadores pueden investigar procesos críticos como la activación de las células inmunitarias, los mecanismos de entrada viral y las complejas vías de señalización que rigen las funciones celulares. Las modificaciones postraduccionales de CD81 son especialmente importantes para comprender el papel de CD81 en condiciones patológicas como la metástasis del cáncer y los trastornos inmunológicos, lo que convierte al anticuerpo anti-CD81 (5A6) en una herramienta esencial tanto para la investigación básica como aplicada.

Para uso exclusivo en Investigación No está diseñado para para Diagnóstico ó Terapia.

Alexa Fluor® es una marca registrada de Molecular Probes Inc., OR., USA

REIVEW LI-COR® y Odyssey® son marcas registradas de LI-COR Biosciences.

Información sobre pedidos

Nombre del productoNúmero de catálogoUNIDADPrecioCANTIDADFavoritos

CD81 Anticuerpo (5A6)

sc-23962
200 µg/ml
$316.00

Paquete de CD81 (5A6): m-IgG Fc BP-HRP

sc-526643
200 µg Ab; 10 µg BP
$354.00

Paquete de CD81 (5A6): m-IgG1 BP-HRP

sc-532016
200 µg Ab; 20 µg BP
$354.00

CD81 Anticuerpo (5A6) AC

sc-23962 AC
500 µg/ml, 25% agarose
$416.00

CD81 Anticuerpo (5A6) HRP

sc-23962 HRP
200 µg/ml
$316.00

CD81 Anticuerpo (5A6) FITC

sc-23962 FITC
200 µg/ml
$330.00

CD81 Anticuerpo (5A6) PE

sc-23962 PE
200 µg/ml
$343.00

CD81 Anticuerpo (5A6) Alexa Fluor® 488

sc-23962 AF488
200 µg/ml
$357.00

CD81 Anticuerpo (5A6) Alexa Fluor® 546

sc-23962 AF546
200 µg/ml
$357.00

CD81 Anticuerpo (5A6) Alexa Fluor® 594

sc-23962 AF594
200 µg/ml
$357.00

CD81 Anticuerpo (5A6) Alexa Fluor® 647

sc-23962 AF647
200 µg/ml
$357.00

CD81 Anticuerpo (5A6) Alexa Fluor® 680

sc-23962 AF680
200 µg/ml
$357.00

CD81 Anticuerpo (5A6) Alexa Fluor® 790

sc-23962 AF790
200 µg/ml
$357.00

What is the isoelectric point for CD-81 antibody?

Preguntado por: Sumita
Thank you for your question. We do not characterize the isoelectric point of our antibodies.
Respondida por: BlakeJ
Fecha de publicación: 2025-07-23

Hello, I have used the CD81 antibody for western blot at 1:1500 in 5% MILK on exosomes from human plasma. Do you know why I get two bands, one at 23 kDa and another one at 48 kDa? Many thanks Juliana

Preguntado por: Juliana Redondo
Thank you for your question. The expected molecular weight for CD81 is ~ 22-26 kDa. We have not tested this antibody specifically in human plasma, and have not observed a higher molecular weight signal at 48 kDa in the cell lines tested. CD81 has been reported to dimerize (PMID: 20375010) and that may be a consideration. We would also recommend including a secondary antibody control, where you omit the primary antibody from the incubation, to confirm if the higher molecular weight band might be due to non-specific binding of the secondary antibody alone.
Respondida por: Technical Support
Fecha de publicación: 2017-10-16

Hi, I have used this antibody at 1:100 concentration on a western blot (10% SDS-PAGE gel) loading some exosomal protein. Could you explain please why I get not only the 26 kDa band, but also a band at about 50 kDa? Thank you.

Preguntado por: VivSal
Thank you for your question. We have not tested this antibody on exosomal protein, or observed a 50 kDa band in Western blot. We would recommend including a secondary antibody control (omit primary antibody) to identify if the 50 kDa band may be a result of non-specific binding of the secondary antibody alone. It could also be your secondary antibody binding to heavy chain IgG in your samples. Below is a link to our recommended protocol for Western blotting. Please contact our Technical Service Department if for additional support with troubleshooting the antibody. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Respondida por: Technical Support
Fecha de publicación: 2017-10-16

I have tried using this antibody on a western blot (after running an SDS-PAGE gel) with a homemade K562 lysate as control and did not get a signal at 1:100 dilution. I tried it 3 times previously on other cell lines at 1:200 w/no success. Any suggestions?

Preguntado por: Tali
Thank you for your question. If you have no signal, we would recommend verifying that your anti-mouse secondary is working well. We would also recommend including a loading control antibody (e.g., Actin or GAPDH) to ensure that your transfer conditions are good, as well as your signal detection conditions (e.g., chemiluminescence or other). We have successfully used this antibody for WB at a dilution of 1:200, loading 50 µg of K-562 whole cell lysate. A link to our complete recommended protocol is provided below. If you continue to have negative results, please contact our Technical Service Department so we can provide further troubleshooting support. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting .
Respondida por: Technical Support
Fecha de publicación: 2017-04-26

What concentration would you recommend using SC-20048 at, to detect a protein in paraffin-embedded human tissue? We have used 1:1000 successfully for western blots. Please can you reply at your earliest convenience.

Preguntado por: Deborah Goberdhan
Thank you for your question! We recommend a starting dilution of 1:50 for IHC on paraffin embedded tissue sections. This is a starting dilution, so it is possible that you can use it much more diluted! We also recommend performing an antigen retrieval step, ideally sodium citrate with heat. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Respondida por: Tech Service
Fecha de publicación: 2017-02-22

1, What concentration do you recommend using SC-23962 on paraffin embedded human tissue sections? We have this antibody, so would like to try. ; 2. Do you think that SC-23962 FITC would work better and at what conc. for same application, ie IHC(P)?

Preguntado por: Deborah Goberdhan
Thank you for your question! We recommend a starting dilution of 1:50 for IHC on paraffin embedded tissue sections. We do recommend performing an antigen retrieval step. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining You can use the FITC conjugated form, although we do not generally use FITC conjugated antibodies for IHC in house, so I do not have test results for this. If you wish to try it, I would stick with the 1:50 starting dilution as well. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Respondida por: Tech Service
Fecha de publicación: 2017-02-22

Can I use this antibody for staining of mouse tissue, since it is a mouse antibody?

Preguntado por: Anna99
Yes. CD81 Antibody (5A6): sc-23962 is available conjugated to either phycoerythrin (sc-23962 PE) or fluoroscein (sc-23962 FITC), 200 µg/ml, for IF, IHC(P) and FCM.
Respondida por: Tech Service 5
Fecha de publicación: 2017-01-05
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Rated 4 de 5 por de Great antibodyI request a sample of the antibody and it worked well!
Fecha de publicación: 2023-07-31
Rated 4 de 5 por de buon funzionamentoHo effettuato un solo esperimento per verificare la reattività nel cane e ha funzionato
Fecha de publicación: 2023-07-14
Rated 1 de 5 por de WBでバンドが検出できませんでした。エクソソームサンプルでCD81のバンドが全く検出できませんでした。同じサンプルで他のCD81抗体を使うとCD81を検出できたのでサンプルは問題ないと考えています。
Fecha de publicación: 2021-09-09
Rated 2 de 5 por de No band in Western blotI cannot see any band in Western blot after a nice transfer (all MW completely transfered; blotting using 1:200 of this antibody and 2nd antibody sc-516102 at 1:1000) of exosomes isolated by ultracentrifugation and further separated by density centrifugation. Perhaps I have low concentration of exosomes, but I expected to get at least a faint band.
Fecha de publicación: 2021-05-25
Rated 5 de 5 por de Works well in western blotThe antibody detected CD81 in Mesenchymal stem cells at the expected molecular size.
Fecha de publicación: 2020-09-18
Rated 5 de 5 por de Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and shown a very strong signal, even at dilution 1:100
Fecha de publicación: 2018-11-19
Rated 5 de 5 por de Excellent antibody for ICC stainingThis antibody is gorgeous for ICC staining, please see the attached confocal image. Highly recommended. I tried 1:50 dilution for the first time, and you may further dilute it to 1:100 or even 1:200 to decrease the background a little bit, and it should work even better. I feel the overall quality of SCBT antibodies is getting much better,
Fecha de publicación: 2018-11-08
Rated 5 de 5 por de likeMembrane localization observed by Immunofluorescence staining of methanol-fixed embedded HeLa cells, also good for staining.
Fecha de publicación: 2017-05-07
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CD81 Anticuerpo (5A6) clasificado 4.1 de 5 por 14.
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