CD71/TFRC/Transferrin Receptor Antibody (YTA 74.4): sc-59112

CD71 Antibody (YTA 74.4) is a rat monoclonal IgG2a antibody that recognizes CD71 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD71 (YTA 74.4) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD71, also known as transferrin receptor (TFR), is a type II membrane glycoprotein essential for iron homeostasis through receptor-mediated endocytosis. This process supports DNA synthesis and cellular proliferation, as iron serves as a crucial cofactor for many enzymes. CD71 shows high expression in proliferating cells, reticulocytes, and erythroid precursors, with increased levels in activated lymphocytes, monocytes, and macrophages during immune responses. CD71 expression extends to most dividing cells and brain endothelium, indicating broad physiological significance. A second transferrin receptor, TFR2, also facilitates transferrin-bound iron uptake, primarily in the liver, contributing to iron regulation. TFR2 gene mutations can cause hereditary hemochromatosis type III (HFE3), an iron overload disorder common among Caucasians, highlighting CD71′s importance in iron metabolism and related diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD71/TFRC/Transferrin Receptor Antibody (YTA 74.4) References:

1. CD71 is selectively and ubiquitously expressed at high levels in erythroid precursors of all maturation stages: a comparative immunochemical study with glycophorin A and hemoglobin A.  |  Dong, HY., et al. 2011. Am J Surg Pathol. 35: 723-32. PMID: 21415701
2. Overexpression of transferrin receptor CD71 and its tumorigenic properties in esophageal squamous cell carcinoma.  |  Chan, KT., et al. 2014. Oncol Rep. 31: 1296-304. PMID: 24435655
3. CD71 targeting boosts immunogenicity of sublingually delivered influenza haemagglutinin antigen and protects against viral challenge in mice.  |  Mann, JF., et al. 2016. J Control Release. 232: 75-82. PMID: 27094605
4. Expression of CD71 by flow cytometry in acute leukemias: More often seen in acute myeloid leukemia.  |  Pande, A., et al. 2016. Indian J Pathol Microbiol. 59: 310-3. PMID: 27510666
5. Umbilical cord CD71+ erythroid cells are reduced in neonates born to women in spontaneous preterm labor.  |  Gomez-Lopez, N., et al. 2016. Am J Reprod Immunol. 76: 280-4. PMID: 27625200
6. Targeting the Difficult-to-Drug CD71 and MYCN with Gambogic Acid and Vorinostat in a Class of Neuroblastomas.  |  Bishayee, K., et al. 2019. Cell Physiol Biochem. 53: 258-280. PMID: 31313541
7. The transferrin receptor CD71 regulates type II CD38, revealing tight topological compartmentalization of intracellular cyclic ADP-ribose production.  |  Deng, QW., et al. 2019. J Biol Chem. 294: 15293-15303. PMID: 31434741
8. Immunological consequences of extramedullary erythropoiesis: immunoregulatory functions of CD71+ erythroid cells.  |  Elahi, S. and Mashhouri, S. 2020. Haematologica. 105: 1478-1483. PMID: 32354873
9. CD71+ Erythroid Cells in Human Neonates Exhibit Immunosuppressive Properties and Compromise Immune Response Against Systemic Infection in Neonatal Mice.  |  Elahi, S., et al. 2020. Front Immunol. 11: 597433. PMID: 33329589
10. Immunotherapy with CD25/CD71-allodepleted T cells to improve T-cell reconstitution after matched unrelated donor hematopoietic stem cell transplant: a randomized trial.  |  Peggs, KS., et al. 2023. Cytotherapy. 25: 82-93. PMID: 36220712

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD71/TFRC/Transferrin Receptor Antibody (YTA 74.4) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-CD71/TFRC/Transferrin Receptor Antibody (YTA 74.4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD71/TFRC/Transferrin Receptor Antibody (YTA 74.4) Specifications

Alternate Product Name: CD71

Product Clone Name: YTA 74.4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: CD71

Epitope Species: mouse

Product Reactivity/Detection: CD71

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for CD71/TFRC/Transferrin Receptor Antibody (YTA 74.4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD71/TFRC/Transferrin Receptor antibody is CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1): sc-32272.