Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BMAL1 Double Nickase Plasmid (m): sc-419206-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMAL1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BMAL1 Double Nickase Plasmid (m) and BMAL1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Arntl. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BMAL1 Antibody (B-1): sc-365645
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMAL1 Double Nickase Plasmid (m) | sc-419206-NIC | 20 µg | $410.00 |
Arntl encodes the core circadian transcription factor BMAL1, which heterodimerizes with CLOCK to bind E-box elements and drive rhythmic expression of clock-controlled genes. This transcription–translation feedback network coordinates cellular oscillations in metabolism, redox homeostasis, DNA damage responses, and immune signaling across tissues. BMAL1 activity interfaces with pathways including PER/CRY repression, REV-ERB/ROR nuclear receptor loops, and nutrient-sensing programs that tune mitochondrial function and lipid and glucose utilization. Dysregulated ARNTL/BMAL1 signaling has been associated with sleep–wake and behavioral phenotypes in model systems and is frequently studied in the context of metabolic, inflammatory, neurodegenerative, and cancer-related processes where circadian control modulates disease-relevant gene expression programs.
BMAL1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Arntl locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Arntl. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Arntl function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Arntl-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.