beta-catenin Antibody (15B8): sc-53483

β-catenin Antibody (15B8) is a mouse monoclonal IgG1 kappa light chain antibody developed against recombinant full-length β-catenin of chicken origin. β-catenin monoclonal antibody (15B8) effectively detects β-catenin from mouse, rat, human, Arabidopsis, bovine, and canine sources across various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), without cross-reactivity to plakoglobin. Available in non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, anti-β-catenin antibody (15B8) offers versatile solutions for diverse experimental needs. β-Catenin, also known as catenin beta-1 or CTNNB1, is a multifunctional protein that plays a crucial role in cell-cell adhesion and gene transcription regulation. β-Catenin localizes primarily at the plasma membrane, where β-catenin binds to the intracellular domain of cadherins, forming catenin-cadherin complexes essential for maintaining cellular adhesion and tissue architecture. In the absence of Wnt signaling, β-catenin is targeted for degradation in the cytoplasm; however, upon Wnt pathway activation, β-catenin accumulates and translocates to the nucleus, where β-catenin interacts with TCF/LEF transcription factors to regulate gene expression involved in cell proliferation and differentiation. The precise localization of β-catenin within the cell is vital, as β-catenin dysregulation can lead to various pathologies, including cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-beta-catenin Antibody (15B8) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-beta-catenin Antibody (15B8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

beta-catenin Antibody (15B8) Specifications

Alternate Product Name: β-catenin

Product Clone Name: 15B8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: β-catenin

Epitope Species: chicken

Product Reactivity/Detection: β-catenin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and canine

Additional Non-Mammalian Reactivity: Arabadopsis

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for beta-catenin Antibody (15B8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred beta-catenin antibody is beta-catenin Antibody (E-5): sc-7963.