Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
XRN1 CRISPR/Cas9 KO Plasmid (h): sc-401910
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XRN1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the XRN1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XRN1 Antibody (C-1): sc-165985
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XRN1 CRISPR/Cas9 KO Plasmid (h) | sc-401910 | 20 µg | $397.00 |
Overview
XRN1 encodes a highly conserved 5′→3′ exoribonuclease that drives cytoplasmic mRNA turnover by degrading decapped transcripts and clearing RNA decay intermediates. It is a core component of post-transcriptional gene regulation, linking decapping and exonucleolytic decay to pathways controlling mRNA surveillance, stress granule dynamics, and innate immune sensing of aberrant or viral RNAs. Through its role in RNA quality control and remodeling of the transcriptome, XRN1 influences cellular responses to stress and infection as well as broader programs in proliferation and differentiation. Dysregulated RNA decay and impaired XRN1 activity have been associated with altered interferon signaling, neurodevelopmental phenotypes, and cancer-relevant changes in gene expression networks.
XRN1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the XRN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the XRN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the XRN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish XRN1 protein expression.
This CRISPR knockout system enables efficient generation of XRN1-deficient cell models for investigation of XRN1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting XRN1 exon(s) critical for XRN1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple XRN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by XRN1 CRISPR/Cas9 KO Plasmid (h) and XRN1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the XRN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by XRN1 HDR Plasmid (h) and XRN1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by XRN1 homology arms to support homology-directed repair at defined XRN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.