von Willebrand Factor/VWF CRISPR/Cas9 KO Plasmid (m): sc-423690

Overview

Vwf encodes von Willebrand factor (VWF), a large multimeric glycoprotein synthesized by endothelial cells and megakaryocytes and stored in Weibel–Palade bodies and platelet α-granules. VWF mediates platelet adhesion and aggregation under high shear by bridging exposed subendothelial collagen to platelet glycoprotein Ib/IX/V, and it stabilizes coagulation factor VIII in circulation. Its regulated secretion and multimer processing integrate endothelial activation, extracellular matrix interactions, and hemostatic protease networks that coordinate primary hemostasis. Dysregulation of VWF abundance, multimer distribution, or clearance perturbs thromboinflammatory balance and is linked to bleeding and thrombotic phenotypes relevant to vascular injury, inflammation, and endothelial dysfunction models.

von Willebrand Factor/VWF CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Vwf gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Vwf together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Vwf open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish von Willebrand Factor/VWF protein expression.

This CRISPR knockout system enables efficient generation of Vwf-deficient cell models for investigation of von Willebrand Factor/VWF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Vwf exon(s) critical for von Willebrand Factor/VWF function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Vwf genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by von Willebrand Factor/VWF CRISPR/Cas9 KO Plasmid (m) and von Willebrand Factor/VWF CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Vwf locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by von Willebrand Factor/VWF HDR Plasmid (m) and von Willebrand Factor/VWF HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Vwf homology arms to support homology-directed repair at defined Vwf target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.