Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TCP-1 γ CRISPR/Cas9 KO Plasmid (h): sc-403203
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TCP-1 γ CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TCP-1 γ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TCP-1 γ Antibody (F-3): sc-271336
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TCP-1 γ CRISPR/Cas9 KO Plasmid (h) | sc-403203 | 20 µg | $397.00 |
Overview
CCT3 encodes the human chaperonin-containing TCP-1 subunit gamma (TCP-1 γ), a core component of the cytosolic TRiC/CCT complex that drives ATP-dependent folding of a broad set of client proteins. TRiC/CCT supports proteostasis by assisting maturation of cytoskeletal proteins such as actin and tubulin and by influencing folding and stability of signaling and cell-cycle regulators. Through these roles, TCP-1 γ contributes to cytoskeleton organization, protein quality control, and proliferative capacity, with perturbations in chaperonin function linked to proteotoxic stress and altered growth signaling. Dysregulated CCT subunit expression has been reported across multiple disease contexts, supporting its study in pathways relevant to oncogenic transformation and neurodegeneration-associated protein misfolding.
TCP-1 γ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CCT3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CCT3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CCT3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TCP-1 γ protein expression.
This CRISPR knockout system enables efficient generation of CCT3-deficient cell models for investigation of TCP-1 γ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CCT3 exon(s) critical for TCP-1 γ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CCT3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TCP-1 γ CRISPR/Cas9 KO Plasmid (h) and TCP-1 γ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CCT3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TCP-1 γ HDR Plasmid (h) and TCP-1 γ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CCT3 homology arms to support homology-directed repair at defined CCT3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.