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T54 Antibody (A-4): sc-398920

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Datasheets
  • T54 Antibody (A-4) is a mouse monoclonal IgG2a κ T54 antibody provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 150-175 within an internal region of T54 of mouse origin
  • T54 Antibody (A-4) is recommended for detection of T54 of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-T54 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for T54 Antibody (A-4) for WB applications. These reagents are now offered in bundles with T54 Antibody (A-4) (see ordering information below).
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    T54 Antibody (A-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects T54 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). T54 (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. T54 protein, also known as GPKOW (G patch domain and KOW motifs), GPATC5, or GPATCH5, is a potential RNA-binding protein characterized by a central G patch domain and two C-terminal KOW domains, comprising 476 amino acids and belonging to the MOS2 family. T54 is a mammalian homolog of the Arabidopsis thaliana MOS2 nuclear protein, which is essential for innate immunity. The presence of G patch and KOW domains in T54 suggests involvement in RNA processing and regulation, which is crucial for maintaining cellular homeostasis and responding to stress signals. T54 localization in the nucleus indicates a potential role in gene expression regulation, making T54 a significant player in the immune response and cellular function.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    T54 Antibody (A-4) References:

    1. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.  |  Strausberg, RL., et al. 2002. Proc Natl Acad Sci U S A. 99: 16899-903. PMID: 12477932
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).  |  Gerhard, DS., et al. 2004. Genome Res. 14: 2121-7. PMID: 15489334
    3. Towards a proteome-scale map of the human protein-protein interaction network.  |  Rual, JF., et al. 2005. Nature. 437: 1173-8. PMID: 16189514
    4. MOS2, a protein containing G-patch and KOW motifs, is essential for innate immunity in Arabidopsis thaliana.  |  Zhang, Y., et al. 2005. Curr Biol. 15: 1936-42. PMID: 16271871
    5. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.  |  Olsen, JV., et al. 2006. Cell. 127: 635-48. PMID: 17081983
    6. Long-range map of a 3.5-Mb region in Xp11.23-22 with a sequence-ready map from a 1.1-Mb gene-rich interval.  |  Schindelhauer, D., et al. 1996. Genome Res. 6: 1056-69. PMID: 8938429

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    T54 Antibody (A-4)

    sc-398920
    200 µg/ml
    $322.00

    T54 Antibody (A-4): m-IgG Fc BP-HRP Bundle

    sc-530971
    200 µg Ab; 10 µg BP
    $361.00

    T54 Antibody (A-4): m-IgGκ BP-HRP Bundle

    sc-524538
    200 µg Ab, 40 µg BP
    $361.00

    T54 Antibody (A-4): m-IgG2a BP-HRP Bundle

    sc-547646
    200 µg Ab; 10 µg BP
    $361.00

    T54 Antibody (A-4) AC

    sc-398920 AC
    500 µg/ml, 25% agarose
    $424.00

    T54 Antibody (A-4) HRP

    sc-398920 HRP
    200 µg/ml
    $322.00

    T54 Antibody (A-4) FITC

    sc-398920 FITC
    200 µg/ml
    $336.00

    T54 Antibody (A-4) PE

    sc-398920 PE
    200 µg/ml
    $349.00

    T54 Antibody (A-4) Alexa Fluor® 488

    sc-398920 AF488
    200 µg/ml
    $364.00

    T54 Antibody (A-4) Alexa Fluor® 546

    sc-398920 AF546
    200 µg/ml
    $364.00

    T54 Antibody (A-4) Alexa Fluor® 594

    sc-398920 AF594
    200 µg/ml
    $364.00

    T54 Antibody (A-4) Alexa Fluor® 647

    sc-398920 AF647
    200 µg/ml
    $364.00

    T54 Antibody (A-4) Alexa Fluor® 680

    sc-398920 AF680
    200 µg/ml
    $364.00

    T54 Antibody (A-4) Alexa Fluor® 790

    sc-398920 AF790
    200 µg/ml
    $364.00

    T54 (A-4) Neutralizing Peptide

    sc-398920 P
    100 µg/0.5 ml
    $69.00

    What application is the blocking peptide sc-398920 P appropriate for?

    Asked by: Randy McDonald
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-03-01
    • y_2026, m_5, d_24, h_14CST
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    Rated 5 out of 5 by from Good antibodyAntibody works well - clear, specific band for western blot with 25 ug U87 cell lysates at 1:1000 primary antibody dilution.
    Date published: 2017-09-11
    Rated 5 out of 5 by from Nice Western Blot data of T54 expression in AMJ2Nice Western Blot data of T54 expression in AMJ2-C8, NIH/3T3 and PC-12 whole cell lysates and mouse liver tissue extract . -SCBT QC
    Date published: 2015-05-19
    Rated 5 out of 5 by from Good Western Blot data of T54 expression in A549Good Western Blot data of T54 expression in A549, A2058, HeLa and U-251-MG whole cell lysates and human liver tissue extract . -SCBT QC
    Date published: 2013-07-11
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    T54 Antibody (A-4) is rated 5.0 out of 5 by 3.
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