SUMF1 Antibody (B-9): sc-376035
- SUMF1 Antibody (B-9) is a mouse monoclonal IgG1 κ SUMF1 antibody provided at 200 µg/ml
- raised against amino acids 225-355 mapping near the C-terminal of SUMF1 of human origin
- SUMF1 Antibody (B-9) is recommended for detection of SUMF1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SUMF1 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SUMF1 Antibody (B-9) for WB applications. These reagents are now offered in bundles with SUMF1 Antibody (B-9) (see ordering information below).
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SUMF1 Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects SUMF1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SUMF1 (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SUMF1, also known as sulfatase modifying factor 1 or FGE, is a crucial protein consisting of 374 amino acids that plays a vital role in the activation of sulfatases, which are enzymes responsible for removing sulfate residues from substrates through the hydrolysis of sulfate esters. The proper functioning of sulfatases depends on the presence of Cα-formylglycine (FGly), a unique amino acid generated post-translationally from cysteine residues by specific enzymes. SUMF1 is predominantly localized in the lumen of the endoplasmic reticulum and is expressed widely, with the highest levels found in the liver, kidney, and pancreas. SUMF1 can exist as a monomer, homodimer, or heterodimer (with SUMF2), and primarily functions to oxidize sulfatase cysteine residues to form the active FGly residue, thereby significantly enhancing sulfatase activity. Deficiencies in the SUMF1 gene can lead to multiple sulfatase deficiency (MSD), a complex disorder associated with various severe symptoms, including metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration, and developmental delays, underscoring the importance of SUMF1 in human health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SUMF1 Antibody (B-9) References:
- The multiple sulfatase deficiency gene encodes an essential and limiting factor for the activity of sulfatases. | Cosma, MP., et al. 2003. Cell. 113: 445-56. PMID: 12757706
- Sulphatase activities are regulated by the interaction of sulphatase-modifying factor 1 with SUMF2. | Zito, E., et al. 2005. EMBO Rep. 6: 655-60. PMID: 15962010
- SUMF1 enhances sulfatase activities in vivo in five sulfatase deficiencies. | Fraldi, A., et al. 2007. Biochem J. 403: 305-12. PMID: 17206939
- Sulfatase modifying factor 1 trafficking through the cells: from endoplasmic reticulum to the endoplasmic reticulum. | Zito, E., et al. 2007. EMBO J. 26: 2443-53. PMID: 17446859
- Multiple sulfatase deficiency is due to hypomorphic mutations of the SUMF1 gene. | Annunziata, I., et al. 2007. Hum Mutat. 28: 928. PMID: 17657823
- Heterozygous deletion of ITPR1, but not SUMF1, in spinocerebellar ataxia type 16. | Iwaki, A., et al. 2008. J Med Genet. 45: 32-5. PMID: 17932120
- Molecular analysis of SUMF1 mutations: stability and residual activity of mutant formylglycine-generating enzyme determine disease severity in multiple sulfatase deficiency. | Schlotawa, L., et al. 2008. Hum Mutat. 29: 205. PMID: 18157819
- Multistep, sequential control of the trafficking and function of the multiple sulfatase deficiency gene product, SUMF1 by PDI, ERGIC-53 and ERp44. | Fraldi, A., et al. 2008. Hum Mol Genet. 17: 2610-21. PMID: 18508857
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SUMF1 Antibody (B-9) | sc-376035 | 200 µg/ml | $322.00 | |||
SUMF1 Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-529808 | 200 µg Ab; 10 µg BP | $361.00 | |||
SUMF1 Antibody (B-9): m-IgGκ BP-HRP Bundle | sc-522828 | 200 µg Ab, 40 µg BP | $361.00 | |||
SUMF1 Antibody (B-9): m-IgG1 BP-HRP Bundle | sc-543699 | 200 µg Ab; 20 µg BP | $361.00 | |||
SUMF1 Antibody (B-9) AC | sc-376035 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SUMF1 Antibody (B-9) HRP | sc-376035 HRP | 200 µg/ml | $322.00 | |||
SUMF1 Antibody (B-9) FITC | sc-376035 FITC | 200 µg/ml | $336.00 | |||
SUMF1 Antibody (B-9) PE | sc-376035 PE | 200 µg/ml | $349.00 | |||
SUMF1 Antibody (B-9) Alexa Fluor® 488 | sc-376035 AF488 | 200 µg/ml | $364.00 | |||
SUMF1 Antibody (B-9) Alexa Fluor® 546 | sc-376035 AF546 | 200 µg/ml | $364.00 | |||
SUMF1 Antibody (B-9) Alexa Fluor® 594 | sc-376035 AF594 | 200 µg/ml | $364.00 | |||
SUMF1 Antibody (B-9) Alexa Fluor® 647 | sc-376035 AF647 | 200 µg/ml | $364.00 | |||
SUMF1 Antibody (B-9) Alexa Fluor® 680 | sc-376035 AF680 | 200 µg/ml | $364.00 | |||
SUMF1 Antibody (B-9) Alexa Fluor® 790 | sc-376035 AF790 | 200 µg/ml | $364.00 |