Rad51 Antibody (G-9): sc-377467
- Rad51 Antibody (G-9) is a mouse monoclonal IgG1 κ, cited in 15 publications, provided at 200 µg/ml
- raised against amino acids 1-92 of Rad51 of human origin
- Anti-Rad51 Antibody (G-9) is recommended for detection of Rad51 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Rad51 Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Rad51 Antibody (G-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with Rad51 Antibody (G-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Rad51 Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody engineered to target the N-terminal region (amino acids 1-92) of the human Rad51 protein, ensuring precise detection across multiple applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) in mouse, rat, and human samples. Rad51, also called Reca and Rad51a, is a critical protein involved in the homologous recombination repair (HRR) pathway, which is essential for the accurate repair of DNA double-strand breaks, maintaining genomic stability, and preventing mutations that can lead to cancer. Functionally, Rad51 facilitates the search for homologous DNA sequences and promotes strand invasion and exchange, forming nucleoprotein filaments that are pivotal for the repair process. This activity is vital for cellular resistance to DNA-damaging agents and for the maintenance of genomic integrity during cell division. Rad51 interacts with key proteins such as BRCA1 and BRCA2, which are integral to the DNA damage response and repair mechanisms. These interactions are crucial for the proper localization and function of Rad51 within the nucleus. Additionally, Rad51 collaborates with other Rad52 family members, including Rad52, Rad54, and the Rad51 paralogs, to coordinate the repair process and ensure efficient resolution of DNA lesions. The ability of Rad51 to form stable filaments and regulation through various post-translational modifications, such as phosphorylation and ubiquitination, are essential for DNA repair and cellular survival. Rad51 (G-9) antibody does not cross-react with other members of the Rad51 family, providing high specificity and reliability for researchers studying DNA repair mechanisms, cancer biology, and genomic stability. Available in both non-conjugated form and various conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, anti-Rad51 antibody (G-9) offers versatile solutions to meet diverse experimental needs.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rad51 Antibody (G-9) References:
- BRCA1-BARD1 promotes RAD51-mediated homologous DNA pairing. | Zhao, W., et al. 2017. Nature. 550: 360-365. PMID: 28976962
- Rad51 filament dynamics and its antagonistic modulators. | Carver, A. and Zhang, X. 2021. Semin Cell Dev Biol. 113: 3-13. PMID: 32631783
- RAD51 Gene Family Structure and Function. | Bonilla, B., et al. 2020. Annu Rev Genet. 54: 25-46. PMID: 32663049
- RAD51 is essential for spermatogenesis and male fertility in mice. | Qin, J., et al. 2022. Cell Death Discov. 8: 118. PMID: 35292640
- RAD51 protects human cells from transcription-replication conflicts. | Bhowmick, R., et al. 2022. Mol Cell. 82: 3366-3381.e9. PMID: 36002000
- Centromeres as universal hotspots of DNA breakage, driving RAD51-mediated recombination during quiescence. | Saayman, X., et al. 2023. Mol Cell. 83: 523-538.e7. PMID: 36702125
- RAD51 bypasses the CMG helicase to promote replication fork reversal. | Liu, W., et al. 2023. Science. 380: 382-387. PMID: 37104614
- RAD51 paralogs synergize with RAD51 to protect reversed forks from cellular nucleases. | Guh, CL., et al. 2023. Nucleic Acids Res. 51: 11717-11731. PMID: 37843130
- Structural basis for stabilisation of the RAD51 nucleoprotein filament by BRCA2. | Appleby, R., et al. 2023. Nat Commun. 14: 7003. PMID: 37919288
- RAD51 restricts DNA over-replication from re-activated origins. | Muñoz, S., et al. 2024. EMBO J. 43: 1043-1064. PMID: 38360996
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad51 Antibody (G-9) | sc-377467 | 200 µg/ml | $322.00 | |||
Rad51 Antibody (G-9): m-IgGκ BP-HRP Bundle | sc-523339 | 200 µg Ab, 40 µg BP | $361.00 | |||
Rad51 Antibody (G-9) AC | sc-377467 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rad51 Antibody (G-9) HRP | sc-377467 HRP | 200 µg/ml | $322.00 | |||
Rad51 Antibody (G-9) FITC | sc-377467 FITC | 200 µg/ml | $336.00 | |||
Rad51 Antibody (G-9) PE | sc-377467 PE | 200 µg/ml | $349.00 | |||
Rad51 Antibody (G-9) Alexa Fluor® 488 | sc-377467 AF488 | 200 µg/ml | $364.00 | |||
Rad51 Antibody (G-9) Alexa Fluor® 546 | sc-377467 AF546 | 200 µg/ml | $364.00 | |||
Rad51 Antibody (G-9) Alexa Fluor® 594 | sc-377467 AF594 | 200 µg/ml | $364.00 | |||
Rad51 Antibody (G-9) Alexa Fluor® 647 | sc-377467 AF647 | 200 µg/ml | $364.00 | |||
Rad51 Antibody (G-9) Alexa Fluor® 680 | sc-377467 AF680 | 200 µg/ml | $364.00 | |||
Rad51 Antibody (G-9) Alexa Fluor® 790 | sc-377467 AF790 | 200 µg/ml | $364.00 |