Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PABP CRISPR/Cas9 KO Plasmid (m): sc-422100
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PABP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PABP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PABP Antibody (10E10): sc-32318
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PABP CRISPR/Cas9 KO Plasmid (m) | sc-422100 | 20 µg | $397.00 |
Overview
Mouse Pabpc1 encodes poly(A)-binding protein (PABP), a central regulator of cytoplasmic mRNA fate that binds poly(A) tails and coordinates translation initiation, mRNA stabilization, and deadenylation-dependent turnover. Through interactions with eIF4G and other RNA-binding proteins, PABP helps couple poly(A) tail length to ribosome recruitment and supports efficient protein synthesis during growth, differentiation, and stress responses. PABP also contributes to post-transcriptional control programs implicated in cell-cycle progression, apoptosis, and innate antiviral responses, making Pabpc1 a relevant node in studies of dysregulated gene expression. Perturbation of PABP-dependent mRNA regulatory networks is commonly examined in the context of oncogenic translation programs and neurodevelopmental or neurodegenerative phenotypes driven by altered RNA metabolism.
PABP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pabpc1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pabpc1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pabpc1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PABP protein expression.
This CRISPR knockout system enables efficient generation of Pabpc1-deficient cell models for investigation of PABP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pabpc1 exon(s) critical for PABP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pabpc1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PABP CRISPR/Cas9 KO Plasmid (m) and PABP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pabpc1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PABP HDR Plasmid (m) and PABP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pabpc1 homology arms to support homology-directed repair at defined Pabpc1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.