p22-phox Double Nickase Plasmid (h): sc-400325-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p22-phox Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- p22-phox Double Nickase Plasmid (h) and p22-phox Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CYBA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p22-phox Antibody (E-11): sc-271968
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p22-phox Double Nickase Plasmid (h) | sc-400325-NIC | 20 µg | $410.00 | |||
p22-phox Double Nickase Plasmid (h2) | sc-400325-NIC-2 | 20 µg | $410.00 |
CYBA encodes p22-phox, a membrane-spanning subunit of the phagocyte NADPH oxidase (NOX2) complex that is required for proper assembly and stabilization of the catalytic gp91-phox/NOX2 core and associated cytosolic factors. By supporting electron transfer to molecular oxygen, p22-phox enables regulated reactive oxygen species (ROS) generation that contributes to antimicrobial defense, redox signaling, and inflammatory responses. CYBA also interfaces with other NOX family enzymes, linking it to broader ROS-dependent pathways that influence endothelial function, innate immune signaling, and oxidative stress responses. Genetic or functional disruption of CYBA is associated with impaired oxidative burst phenotypes and has been studied in the context of immunodeficiency, chronic inflammation, and ROS-driven tissue injury mechanisms.
p22-phox Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CYBA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CYBA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CYBA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CYBA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.