p21 Waf1/Cip1/CDKN1A Antikörper (F-5) Empfohlen für die Detektion von p21 Waf1/Cip1 aus der Spezies mouse, rat und human per WB, IP, IF, IHC(P) und FCM

p21 Waf1/Cip1/CDKN1A Antikörper (F-5): sc-6246

p21 Waf1/Cip1/CDKN1A Antikörper (F-5) wurde bewertet mit 4.4 von 5 von 90.
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Datenblatt

    • Anti-p21 Waf1/Cip1/CDKN1A Antikörper F-5 ist ein monoklonales IgG2b κ p21 Waf1/Cip1/CDKN1A Antikörper, verwendet in 1.508 wissenschaftlichen Veröffentlichungen, in einer Menge von 200 µg/ml
    • gezogen gegen Aminosäuren 1-159, die das Volllänge- p21 Waf1/Cip1 aus der Spezies mouse darstellen
    • Anti-p21 Waf1/Cip1/CDKN1A Antikörper (F-5) ist empfohlen für die Detektion von p21 Waf1/Cip1 aus der Spezies mouse, rat und human per WB, IP, IF, IHC(P) und FCM
    • Anti-p21 Waf1/Cip1/CDKN1A Antikörper (F-5) ist erhältlich als Konjugat mit Agarose für IP; HRP für WB, IHC(P) und ELISA; und entweder mit Phycoerythrin oder FITC für IF, IHC(P) und FCM
    • auch erhältlich als Konjugat mit Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 oder Alexa Fluor® 647 für IF, IHC(P) und FCM
    • auch erhältlich als Konjugat mit Alexa Fluor® 680 oder Alexa Fluor® 790 für WB (NIR), IF und FCM
    • auch konjugiert erhältlich mit Biotin für WB, IHC(P) und ELISA; und entweder mit TRITC oder Alexa Fluor® 405 für IF, IHC(P) und FCM
    • Bitte kontaktieren Sie uns um ein 10 µg GRATIS Sample vom p21 Waf1/Cip1/CDKN1A (F-5): sc-6246 zu erhalten.
    • m-IgG Fc BP-HRP (Maus IgG Fc Bindeprotein-HRP) ist das empfohlene Detektionsreagenz für p21 Waf1/Cip1/CDKN1A Antikörper (F-5) für WB and IHC(P) Experimente. Dieses Reagenz wird nun auch als Bundle angeboten, zusammen mit p21 Waf1/Cip1/CDKN1A Antikörper (F-5) (Bestellinformation unten stehend).

What is the recommended antigen retrieval protocol for IHC-P using this antibody?

Gefragt von: nmurao
Thank you for your question. You can find our recommended antigen retrieval method in our standard protocol which is available under this link: https://www.scbt.com/de/resources/protocols/immunoperoxidase-staining
Beantwortet von: Tech Support Europe
Veröffentlichungsdatum: 2020-09-28

I'm having issues with the detection of p21 wih this antibody in A549 cells treated with nutlin or etoposide. Can you recommend western blot conditions for this cell line and protein? I'm using 1:200 dilution in TBST milk. Thanks

Gefragt von: eabd2
Thank you for your question. We are sorry to hear that this antibody isn't performing as expected. We want you to be successful in your research! Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody.
Beantwortet von: Tech Service
Veröffentlichungsdatum: 2019-11-14

What is the recommended blocking solution for p21 ab in WB?

Gefragt von: Sonia S
Tack för din fråga! We recommend blocking in 5% milk and TBS-T for Western blot with our p21 antibodies.
Beantwortet von: Tech Service
Veröffentlichungsdatum: 2019-08-28

I used this p21 antibody for Western blotting of both cell sample and tissue sample. For cell sample, the band is at 21 kD; but for tissue, the band is at around 25 kD. Is that normal? What is the probable explanation of it?

Gefragt von: Liuershiba
Thank you for your question. The difference in observed molecular weight in your samples could be due to post-translational modification of the p21 protein in the tissue that is not present in the cells. However, inclusion of proper positive and negative controls is always recommended.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2019-06-16

If I am using this antibody to stain P21 positive cells in the brain via immunofluorescence, what secondary antibody should I use?

Gefragt von: khanson
Thank you for your question. p21 Antibody (F-5) is a mouse monoclonal IgG2b, so we would recommend using a secondary antibody that is anti-mouse IgG, or anti-mouse IgG2b for detection. One example for immunofluorescence is our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 488 (CFL 488), m-IgGκ BP-CFL 488: sc-516176.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2019-04-20

p21 Antibody (F-5): sc-6246  Is this antibody work for Rhesus monkey?

Gefragt von: Fei38750
Thank you for your question. We are aware of several product citations using this antibody in monkey, including Vero cells. However, nothing specifically in Rhesus macaque.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2019-04-13

I've recently got the sample F-15 antibody sample and ran the WB using C2C12, mouse myoblast cells. But all the time, I can see the major bands between 15 and 20kD. I used 15% gel. Is it correct size? The size of this protein is not 21kDa?

Gefragt von: Aiden
Thank you for your question. As you mentioned the expected molecular weight of p21 is 21 kDa, and that is what is expected when using this mouse monoclonal, sc-6246, p21 (F-5). We would recommend including a secondary antibody control for your Western blot, where you incubate the membrane without primary antibody. That would identify any non-specific bands that are the result of your secondary antibody alone. A band observed below 15 kDa would be a concern, but since it is normal to see a variation of +/- 5Kda in a western (could be marker differences, gel uneveness, etc), I believe the band you are seeing is p21. Please contact our Technical Service Department if you would like to discuss this further!
Beantwortet von: Tech Service
Veröffentlichungsdatum: 2017-07-05

Is it possible to provide the MSDS fiche for this product?

Gefragt von: BDCC
Thank you for your question. We do not provide MSDSs for our antibody products, but please contact our European office and they will be glad to help get you the information you need. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Beantwortet von: Tech Service
Veröffentlichungsdatum: 2017-06-09
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Rated 5 von 5 von aus 功能很出色! 我一个月前购买了此产品,并为此感到高兴,下次有机会还会来购买!另外圣克鲁兹的产品价格便宜实惠,实验效果好,实在是太棒了!
Veröffentlichungsdatum: 2020-11-20
Rated 3 von 5 von aus è cross reattivo nel cane ma non nel cavallo non è cross reattivo nella specie equina, è comunque cross reattivo nel cane
Veröffentlichungsdatum: 2020-10-07
Rated 3 von 5 von aus For WB We got a free sample of this antibody for WB. This antibody could detect p21 of p21 transfected 293T cell lysate easily. We will use this antibody for WB from now on.
Veröffentlichungsdatum: 2019-07-17
Rated 2 von 5 von aus Immunofluorescence on mouse endothelial cells I tested the antibody in immunofluorescence and I obtained nuclear staining of only few cells.
Veröffentlichungsdatum: 2019-04-28
Rated 5 von 5 von aus Good Ab for IF This antibody is good for immunofluorescent staining (1:50 in 1%BSA in PBS). Highly recommended!
Veröffentlichungsdatum: 2018-11-13
Rated 5 von 5 von aus Very good anti-p21 in western blot Very good anti-p21 in western blot at 1/1000 dilution in TBS-tween 5% milk
Veröffentlichungsdatum: 2018-10-03
Rated 5 von 5 von aus good product Good antibody with clean background for Western Blot.
Veröffentlichungsdatum: 2018-09-11
Rated 5 von 5 von aus Expected result! I got a free sample of p21 (F-5): sc-6246 for carrying out a western blot analysis. Results were as espected with a clear and specific band. I am very happy with this antibody.
Veröffentlichungsdatum: 2018-09-03
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