p-CD3-zeta Antibody (96.Tyr 111): sc-135759
- p-CD3-ζ Antibody (96.Tyr 111) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against a short amino acid sequence containing phosphorylated Tyr 111 of CD3-ζ of human origin
- recommended for detection of Tyr 111 phosphorylated CD3-Ω of mouse, rat and human origin by WB, IP and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p-CD3-ζ Antibody (96.Tyr 111) for WB applications. These reagents are now offered in bundles with p-CD3-ζ Antibody (96.Tyr 111) (see ordering information below).
p-CD3-ζ Antibody (96.Tyr 111) is a mouse monoclonal IgG1 antibody that detects p-CD3-ζ in mouse, rat, and human samples through applications such as western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). p-CD3-ζ plays a crucial role in T cell activation by forming part of the CD3 complex, which is essential for transmitting signals from the T cell receptor upon antigen recognition. p-CD3-ζ is primarily located in the plasma membrane of T cells, where p-CD3-ζ associates with the T cell receptor and other signaling molecules. p-CD3-ζ presence at the cell surface is vital for the initiation of T cell responses, as p-CD3-ζ helps to propagate the activation signal that leads to T cell proliferation and differentiation. Additionally, p-CD3-ζ contains immunoreceptor tyrosine-based activation motifs (ITAMs) that are critical for recruiting and activating downstream signaling proteins, thereby amplifying the immune response. p-CD3-ζ interaction with various kinases, such as ZAP-70, underscores p-CD3-ζ importance in the signaling cascade that ultimately results in T cell activation and function. anti-p-CD3-ζ antibody (96.Tyr 111) is an invaluable tool for researchers studying T cell biology and immune responses.
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p-CD3-zeta Antibody (96.Tyr 111) References:
- DAP12 and KAP10 (DAP10)-novel transmembrane adapter proteins of the CD3zeta family. | Wilson, MJ., et al. 2000. Immunol Res. 22: 21-42. PMID: 10945225
- Imaging T-cell receptor activation reveals accumulation of tyrosine-phosphorylated CD3ζ in the endosomal compartment. | Yudushkin, IA. and Vale, RD. 2010. Proc Natl Acad Sci U S A. 107: 22128-33. PMID: 21135224
- TCR crosslinking promotes Crk adaptor protein binding to tyrosine-phosphorylated CD3ζ chain. | Dong, G., et al. 2017. Biochem Biophys Res Commun. 488: 541-546. PMID: 28526413
- T cells engineered with full-length NKG2D linked to signaling domains of 4-1BB and CD3ζ show enhanced antitumor activity. | Teng, X., et al. 2023. Immunol Cell Biol. 101: 458-464. PMID: 36811384
- INPP5E regulates CD3ζ enrichment at the immune synapse by phosphoinositide distribution control. | Chiu, TY., et al. 2023. Commun Biol. 6: 911. PMID: 37670137
- CD3ζ ITAMs enable ligand discrimination and antagonism by inhibiting TCR signaling in response to low-affinity peptides. | Gaud, G., et al. 2023. Nat Immunol. 24: 2121-2134. PMID: 37945821
- Integration of ζ-deficient CARs into the CD3ζ gene conveys potent cytotoxicity in T and NK cells. | Kath, J., et al. 2024. Blood. 143: 2599-2611. PMID: 38493479
- Exploitation of CD3ζ to enhance TCR expression levels and antigen-specific T cell function. | Degirmencay, A., et al. 2024. Front Immunol. 15: 1386132. PMID: 38873603
- Specific interaction of the CD45 protein-tyrosine phosphatase with tyrosine-phosphorylated CD3 zeta chain. | Furukawa, T., et al. 1994. Proc Natl Acad Sci U S A. 91: 10928-32. PMID: 7526385
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-CD3-ζ Antibody (96.Tyr 111) | sc-135759 | 200 µg/ml | $322.00 | |||
p-CD3-ζ Antibody (96.Tyr 111): m-IgG Fc BP-HRP Bundle | sc-539883 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-CD3-ζ Antibody (96.Tyr 111): m-IgG1 BP-HRP Bundle | sc-541761 | 200 µg Ab; 20 µg BP | $361.00 |