NOXA Antibody (F-3): sc-515840
- NOXA Antibody (F-3) is a mouse monoclonal IgG1 κ, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 1-54 representing full length NOXA of human origin
- NOXA Antibody (F-3) is recommended for detection of NOXA of human origin by WB, IP, IF and ELISA
- Anti-NOXA Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NOXA Antibody (F-3) for WB applications. These reagents are now offered in bundles with NOXA Antibody (F-3) (see ordering information below).
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NOXA Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects NOXA of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NOXA (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NOXA, a pro-apoptotic member of the Bcl-2 family, plays a crucial role in regulating programmed cell death, which is vital for maintaining cellular homeostasis and preventing tumorigenesis. NOXA contains the Bcl-2 homology 3 (BH3) domain, which is essential for promoting apoptosis, while lacking other BH domains present in anti-apoptotic proteins. NOXA is selectively localized to the mitochondria, where NOXA interacts with other Bcl-2 family members to modulate apoptotic signaling pathways. This mitochondrial localization positions NOXA to directly influence the release of cytochrome c and other pro-apoptotic factors, thereby initiating the apoptotic cascade. In murine cells, NOXA mRNA is constitutively expressed at low levels across various organs, with expression levels significantly increasing following X-ray irradiation. In human cells, NOXA, also known as PMA-induced protein 1 or APR, exhibits high expression in the adult T cell leukemia cell line IKD, suggesting a role as an immediate-early-response gene in cellular stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NOXA Antibody (F-3) References:
- Exploiting the pro-apoptotic function of NOXA as a therapeutic modality in cancer. | Guikema, JE., et al. 2017. Expert Opin Ther Targets. 21: 767-779. PMID: 28670929
- Noxa upregulation and 5-gene apoptotic biomarker panel in colorectal cancer. | Kosmidou, V., et al. 2021. Eur J Clin Invest. 51: e13353. PMID: 32682341
- Cullin-5 neddylation-mediated NOXA degradation is enhanced by PRDX1 oligomers in colorectal cancer. | Xu, S., et al. 2021. Cell Death Dis. 12: 265. PMID: 33712558
- NOXA-mediated degradation of MCL1 and BCL2L1 causes apoptosis of daunorubicin-treated human acute myeloid leukemia cells. | Chiou, JT., et al. 2021. J Cell Physiol. 236: 7356-7375. PMID: 33982799
- NOXA Is Important for Verticillium dahliae's Penetration Ability and Virulence. | Zhu, X., et al. 2021. J Fungi (Basel). 7: PMID: 34682235
- NOXA expression drives synthetic lethality to RUNX1 inhibition in pancreatic cancer. | Doffo, J., et al. 2022. Proc Natl Acad Sci U S A. 119: PMID: 35197278
- Identification of NOXA as a pivotal regulator of resistance to CAR T-cell therapy in B-cell malignancies. | Yan, X., et al. 2022. Signal Transduct Target Ther. 7: 98. PMID: 35370290
- Pituitary adenomas evade apoptosis via noxa deregulation in Cushing's disease. | Asuzu, DT., et al. 2022. Cell Rep. 40: 111223. PMID: 36001971
- NOXA Accentuates Apoptosis Induction by a Novel Histone Deacetylase Inhibitor. | Ashry, R., et al. 2023. Cancers (Basel). 15: PMID: 37509312
- Noxa inhibits oncogenesis through ZNF519 in gastric cancer and is suppressed by hsa-miR-200b-3p. | Shi, J., et al. 2024. Sci Rep. 14: 6568. PMID: 38503887
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NOXA Antibody (F-3) | sc-515840 | 200 µg/ml | $322.00 | |||
NOXA Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-531538 | 200 µg Ab; 10 µg BP | $361.00 | |||
NOXA Antibody (F-3): m-IgGκ BP-HRP Bundle | sc-525250 | 200 µg Ab, 40 µg BP | $361.00 | |||
NOXA Antibody (F-3): m-IgG1 BP-HRP Bundle | sc-544610 | 200 µg Ab; 20 µg BP | $361.00 | |||
NOXA Antibody (F-3) AC | sc-515840 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NOXA Antibody (F-3) HRP | sc-515840 HRP | 200 µg/ml | $322.00 | |||
NOXA Antibody (F-3) FITC | sc-515840 FITC | 200 µg/ml | $336.00 | |||
NOXA Antibody (F-3) PE | sc-515840 PE | 200 µg/ml | $349.00 | |||
NOXA Antibody (F-3) Alexa Fluor® 488 | sc-515840 AF488 | 200 µg/ml | $364.00 | |||
NOXA Antibody (F-3) Alexa Fluor® 546 | sc-515840 AF546 | 200 µg/ml | $364.00 | |||
NOXA Antibody (F-3) Alexa Fluor® 594 | sc-515840 AF594 | 200 µg/ml | $364.00 | |||
NOXA Antibody (F-3) Alexa Fluor® 647 | sc-515840 AF647 | 200 µg/ml | $364.00 | |||
NOXA Antibody (F-3) Alexa Fluor® 680 | sc-515840 AF680 | 200 µg/ml | $364.00 | |||
NOXA Antibody (F-3) Alexa Fluor® 790 | sc-515840 AF790 | 200 µg/ml | $364.00 |