Nodal Antibody (WS65): sc-81953

Nodal Antibody (WS65) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nodal in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Nodal antibody (WS65) is available in a non-conjugated form, providing flexibility for various experimental needs. Nodal, a member of the transforming growth factor β (TGFβ) superfamily, plays a crucial role in embryonic development, particularly in the formation of mesoderm during gastrulation. Nodal is expressed in the primitive streak, where ectodermal cells delaminate and differentiate into mesoderm, highlighting its importance in early developmental processes. Additionally, Nodal is involved in the regulation of left-right asymmetry in the developing embryo, influencing the direction of heart looping and overall embryonic turning. Understanding Nodal function is vital, as dysregulation can lead to developmental disorders and congenital anomalies. With broad species reactivity and multiple applications, Nodal monoclonal antibody (WS65) serves as an essential tool for researchers studying developmental biology and related fields.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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Nodal Antibody (WS65) References:

1. The atrioventricular nodal reentrant tachycardia circuit: a proposal.  |  Katritsis, DG. and Becker, A. 2007. Heart Rhythm. 4: 1354-60. PMID: 17905343
2. Nodal expression in the uterus of the mouse is regulated by the embryo and correlates with implantation.  |  Park, CB. and Dufort, D. 2011. Biol Reprod. 84: 1103-10. PMID: 21270430
3. The transforming growth factor-beta family.  |  Massagué, J. 1990. Annu Rev Cell Biol. 6: 597-641. PMID: 2177343
4. Nodal promotes the generation of M2-like macrophages and downregulates the expression of IL-12.  |  Wang, XF., et al. 2014. Eur J Immunol. 44: 173-83. PMID: 24114072
5. Atrioventricular Nodal Reentrant Tachycardia with 2:1 Atrioventricular Block.  |  Larroussi, L. and Badhwar, N. 2016. Card Electrophysiol Clin. 8: 51-5. PMID: 26920169
6. Multiple type-beta transforming growth factors and their receptors.  |  Massagué, J., et al. 1987. J Cell Physiol Suppl. Suppl 5: 43-7. PMID: 2890647
7. Clinical presentation of ventricular-Hisian and ventricular-nodal accessory pathways.  |  Chung, R., et al. 2019. Heart Rhythm. 16: 369-377. PMID: 30103070
8. The primitive streak.  |  Bellairs, R. 1986. Anat Embryol (Berl). 174: 1-14. PMID: 3518538
9. Ultra-high-density mapping for safe and effective typical atrioventricular nodal reentrant tachycardia ablation.  |  Sakamoto, Y. and Osanai, H. 2023. Nagoya J Med Sci. 85: 844-851. PMID: 38155630
10. Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation.  |  Zhou, X., et al. 1993. Nature. 361: 543-7. PMID: 8429908
11. Relationship between asymmetric nodal expression and the direction of embryonic turning.  |  Collignon, J., et al. 1996. Nature. 381: 155-8. PMID: 8610012
12. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member.  |  McPherron, AC., et al. 1997. Nature. 387: 83-90. PMID: 9139826

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nodal Antibody (WS65) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Nodal Antibody (WS65) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nodal Antibody (WS65) Specifications

Alternate Product Name: Nodal

Product Clone Name: WS65

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 275-347

Epitope Target: Nodal

Epitope Species: human

Product Reactivity/Detection: Nodal

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Nodal Antibody (WS65): N/A