Nectin 3 Antibody (N3.2.9): sc-69714

Nectin 3 Antibody (N3.2.9) is a mouse monoclonal IgG2a antibody that detects Nectin 3 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Nectin 3 is a crucial transmembrane protein that plays a significant role in cell adhesion and communication, particularly in the nervous system and during viral infections. Nectin 3 is part of the Nectin family, which includes Nectin 1, 2, and 3, and is characterized by three immunoglobulin-like domains in the extracellular region. Notably, Nectin 3 has three splicing variants: Nectin 3α, 3β, and 3γ, with the first two containing a conserved C-terminal motif that is essential for binding to the PDZ domain of afadin, a protein that connects Nectin 3 to the actin cytoskeleton. This interaction maintains the structural integrity of adherens junctions, where Nectin 3α co-localizes with Nectin 2, facilitating cell-cell adhesion and signaling. Nectin 3′s ability to mediate these interactions underscores its importance in both normal cellular functions and in disease pathogenesis, including its role as a receptor for α herpesviruses, making Nectin 3 a significant target for research and therapeutic applications.

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Nectin 3 Antibody (N3.2.9) References:

1. Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin.  |  Reymond, N., et al. 2000. Gene. 255: 347-55. PMID: 11024295
2. Tage4/Nectin-like molecule-5 heterophilically trans-interacts with cell adhesion molecule Nectin-3 and enhances cell migration.  |  Ikeda, W., et al. 2003. J Biol Chem. 278: 28167-72. PMID: 12740392
3. Tauopathy differentially affects cell adhesion molecules in mouse brain: early down-regulation of nectin-3 in stratum lacunosum moleculare.  |  Maurin, H., et al. 2013. PLoS One. 8: e63589. PMID: 23704923
4. Nectin-3 (CD113) interacts with Nectin-2 (CD112) to promote lymphocyte transendothelial migration.  |  Devilard, E., et al. 2013. PLoS One. 8: e77424. PMID: 24116228
5. The expression of the Nectin complex in human breast cancer and the role of Nectin-3 in the control of tight junctions during metastasis.  |  Martin, TA., et al. 2013. PLoS One. 8: e82696. PMID: 24386110
6. Nectin expression in pancreatic adenocarcinoma: nectin-3 is associated with a poor prognosis.  |  Izumi, H., et al. 2015. Surg Today. 45: 487-94. PMID: 25690753
7. Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells.  |  Xu, F., et al. 2019. Biomed Pharmacother. 110: 139-144. PMID: 30469078
8. Loss of nectin-3 expression as a marker of tumor aggressiveness in pancreatic neuroendocrine tumor.  |  Hirabayashi, K., et al. 2020. Pathol Int. 70: 84-91. PMID: 31855317
9. ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin-3 and NRXN3.  |  Zhao, Y., et al. 2020. Cancer Commun (Lond). 40: 721-737. PMID: 33038291
10. Precise levels of nectin-3 are required for proper synapse formation in postnatal visual cortex.  |  Tomorsky, J., et al. 2020. Neural Dev. 15: 13. PMID: 33160402

The preferred Nectin 3 antibody is Nectin 3 Antibody (H-11): sc-271611.