MyoD Antikörper (G-1) Empfohlen für die Detektion von MyoD aus der Spezies mouse, rat und human per WB, IP, IF, IHC(P) und ELISA

MyoD Antikörper (G-1): sc-377460

MyoD Antikörper (G-1) wurde bewertet mit 4.7 von 5 von 19.
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Datenblatt

    • Anti-MyoD Antikörper G-1 ist ein Maus monoklonales IgG2b κ MyoD Antikörper, verwendet in 68 wissenschaftlichen Veröffentlichungen, in einer Menge von 200 µg/ml
    • gezogen gegen die Aminosäuresequenz 1-318 von MyoD aus der Spezies mouse
    • Anti-MyoD Antikörper (G-1) ist empfohlen für die Detektion von MyoD aus der Spezies mouse, rat und human per WB, IP, IF, IHC(P) und ELISA
    • Anti-MyoD Antikörper (G-1) ist erhältlich als Konjugat mit Agarose für IP; HRP für WB, IHC(P) und ELISA; und entweder mit Phycoerythrin oder FITC für IF, IHC(P) und FCM
    • auch erhältlich als Konjugat mit Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 oder Alexa Fluor® 647 für IF, IHC(P) und FCM
    • auch erhältlich als Konjugat mit Alexa Fluor® 680 oder Alexa Fluor® 790 für WB (NIR), IF und FCM
    • erhältlich als TransCruz Reagenz für Gel Supershift und ChIP (sc-377460 X, 200 µg/0.1 ml)
    • Bitte kontaktieren Sie uns um ein 10 µg GRATIS Sample vom MyoD (G-1): sc-377460 zu erhalten.
    • m-IgG2b BP-HRP und m-IgGκ BP-HRP sind die empfohlenen Detektionsreagenzien für MyoD Antikörper (G-1) für WB and IHC(P) Experimente. Diese Reagenzien werden nun auch als Bundle angeboten, zusammen mit MyoD Antikörper (G-1) (Bestellinformation unten stehend).
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いつもお世話になります。 御社の下記の抗体を使用しています。 MyoD (G-1)  データシート等に掲載されているWBの予測分子量は約45kDaです。 しかしながら、この45kDaに加え、約70kDa近辺に強いバンドを検出しています。 我々の結果の場合、45kDaより70kDaのバンドの方が強く検出されます。 御社のホームページ上でも70kDa近辺に強いバンドが検出されているデータが掲載されています。 この70kDaのバンドの由来に関する情報をお持ちでしたら、ご教示ください。

Gefragt von: Shuji
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2021-07-23

您好,我想问一下,我买的myod的抗体,应该放在四度的,但是放在-20度冰箱24个小时了,请问会有影响吗?十分着急!麻烦你帮我解答一下谢谢!

Gefragt von: 诸葛钢铁
感谢您的提问。MyoD 抗体 (G-1): sc-377460 应该保存在4°C,不能冷冻。冷冻后对产品的质量可能会造成不良影响。 Thank you for your question. MyoD Antibody (G-1): sc-377460 should be stored at 4 °C. Do NOT FREEZE. The Quality of the product may be affected after freezing.
Beantwortet von: Technical Support 12
Veröffentlichungsdatum: 2021-07-07

I had used MyoD C-20 to detect MyoD in chicken myoblasts. It was great. However, C-20 was discontiuned and MyoD G-1 was produced instead. So, I brought the G-1, but the detection was not as good as C-20. Is there any way to get C-20?

Gefragt von: megimetel
Thank you for your question. Unfortunately, the rabbit polyclonal antibody, MyoD (C-20): sc-304, has been discontinued since 2016. It is no longer available for sale or distribution. We do not have any data or product citations yet using this mouse monoclonal antibody in chicken or avian samples. Please contact our Technical Service Department and we could assist you by performing sequence analysis to predict potential cross reactivity of our MyoD antibodies with chicken samples.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2020-03-26

HI, I'm interested in detecting MYOD in bovine cells . do you know if it cross rects? thanks

Gefragt von: Anonym
Thank you for your question. This antibody hasn’t been tested in bovine samples. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined, so we can not perform sequence analysis at this time to predict potential cross reactivity with that species.
Beantwortet von: Tech Service
Veröffentlichungsdatum: 2019-06-28

Buenos días, estoy haciendo una inmunoprecipitacion con MyoD y quiero ver su anticuerpo HDAC1, pero la banda que me sale a 60 kDa no tengo claro si es HDAC1 o es la IgG, podrian decirme el peso al que me deberian salir las IgG??

Gefragt von: Anonym
Muchas gracias por su pregunta. El peso molecular de la IgG es de 150 kDa, la cadena pesada de aprox 50 kDa mientras la ligera de aprox 25 kDa. Si está realizando otro método de detección, puede encontrar todos nuestros protocolos en: https://www.scbt.com/scbt/es/resources/protocols. Si necesita más información, no dude en contactar con nosotros: [email protected]
Beantwortet von: Technical Support Europe
Veröffentlichungsdatum: 2019-02-27

Can I use This antibody in the mouse skeletal muscles?

Gefragt von: sunny22
Thank you for your question. Yes, this antibody has been previously published for the detection of MyoD in mouse skeletal muscle. Please refer to the following publication for additional details: PMID: 28638997.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2019-01-25

The Western shown for this antibody shows a band at close to 55 kd. The western for MyoD 5.8A shows a band between 37 and 43 kd. Both are for MyoD. Please explain this discrepancy.

Gefragt von: SBSPhD
Thank you for your question. The base predicted weight for MyoD determined by sequence is approximately 35 kDa, however the protein appears to go through modification that results in the observed weight running at a variety of higher sizes. Publications have observed weights from 36-38 kDa through 43-45 kDa for the primary band, with some publications also reporting a band around 55-65 kDa. Due to this variability in size observed in various sample types, anything within this range that is clean and consistent is generally accepted. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Beantwortet von: Technical Service
Veröffentlichungsdatum: 2017-02-21

1. In the Western analysis shown for this antibody, you have a positive band showing in at least 3 cell lines that are not myogenic and should not be expressing MyoD1. NIH3T3, 10T1/2, HeLa. Please explain.

Gefragt von: SBSPhD
Thank you for your question. While you are correct that skeletal tissues and other myogenic samples specifically show high levels of MyoD expression, other cell lines and tissue types are not null for the MyoD protein. Most commercial sources have seen positive detection in common cell lines such as HeLa. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Beantwortet von: Technical Service
Veröffentlichungsdatum: 2017-02-21
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Rated 5 von 5 von aus Good antibody We purchased this antibody for immunostainning and western blot. It works very well for both the applications in cultured myoblasts.
Veröffentlichungsdatum: 2018-05-06
Rated 4 von 5 von aus good ab I got a free sample from SCBT, and i tested it on primary satellite cell at 1/50. it worked well for Immunofluorescence.
Veröffentlichungsdatum: 2017-10-13
Rated 5 von 5 von aus Great for pull down assays. Got this antibody for my immunoprecipitation project for human samples. Works great. Western blotting with MyoD western antibody shows clear double bands below 50KD IgG heavy chain band which is commonly detect in human samples. Decided to move on our work with this antibody.
Veröffentlichungsdatum: 2017-09-29
Rated 5 von 5 von aus Great product We used this product for immunostaining in satellite cells. Works well for us.
Veröffentlichungsdatum: 2017-09-27
Rated 5 von 5 von aus Great Antibody I received the free trial and found that this antibody was very specific for the primary myoblast cultures I am working with. Fixed cells with methanol at -20C for 5 minutes and obtained great images.
Veröffentlichungsdatum: 2017-08-03
Rated 4 von 5 von aus Dear I bought it a mouth ago.I think it is very well.The concentration of MyoD is 1:1000.
Veröffentlichungsdatum: 2017-07-05
Rated 4 von 5 von aus It is ok Used at 1:1000 in C2C12 cells. It is ok. There are also some non specific bands there.
Veröffentlichungsdatum: 2017-06-02
Rated 5 von 5 von aus worked well MyoD antibody is suitable for western blot analysis in NIH/3T3 cell, and it worked well.
Veröffentlichungsdatum: 2017-05-24
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