Monomethyl Histone H4 Antibody (5E10-D8): sc-134221

Monomethyl Histone H4 Antibody (5E10-D8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Monomethyl Histone H4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Monomethyl Histone H4 antibody (5E10-D8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Monomethyl Histone H4 plays a crucial role in regulating gene expression and chromatin structure through involvement in transcriptional activation and repression. Methylation of Lys 20 on Histone H4 represents a key post-translational modification influencing chromatin compaction and accessibility, impacting DNA transactions such as replication and repair. This modification maintains proper cellular functions and ensures appropriate gene expression timing and levels, vital for normal development and cellular responses to environmental signals. Monomethyl Histone H4 (5E10-D8) monoclonal antibody′s specific recognition of this modified Histone H4 form makes this reagent invaluable for researchers studying epigenetic regulation and chromatin dynamics in various biological contexts.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Monomethyl Histone H4 Antibody (5E10-D8) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Monomethyl Histone H4 Antibody (5E10-D8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Monomethyl Histone H4 Antibody (5E10-D8) Specifications

Alternate Product Name: Monomethyl Histone H4

Product Clone Name: 5E10-D8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 16-26

Epitope Target: Lys 20 monomethylated Histone H4

Epitope Species: human

Product Reactivity/Detection: Lys 20 monomethylated Histone H4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Monomethyl Histone H4 Antibody (5E10-D8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin