MDMX Antibody (G-10): sc-74467
- MDMX Antibody (G-10) is a mouse monoclonal IgG2a κ MDMX antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 361-490 mapping at the C-terminus of MDMX of human origin
- MDMX Antibody (G-10) is recommended for detection of MDMX of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-MDMX Antibody (G-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for MDMX Antibody (G-10) for WB applications. These reagents are now offered in bundles with MDMX Antibody (G-10) (see ordering information below).
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MDMX Antibody (G-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects MDMX protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MDMX antibody (G-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MDMX protein plays a crucial role in regulating p53 protein stability, which is essential for maintaining cellular integrity and preventing tumorigenesis. MDMX, an MDM2-related protein, inhibits p53 degradation mediated by MDM2 through distinct interactions, thereby influencing p53′s function in cell cycle regulation and apoptosis. MDMX (also known as MDM4) is frequently amplified in malignant gliomas, highlighting its significance in cancer biology. Additionally, MDMX interacts with ARF protein, which sequesters MDMX in the nucleolus, leading to enhanced p53 transactivation and increased p53 activity. This dynamic interplay between MDMX, MDM2, and p53 underscores MDMX′s importance in regulating critical cellular processes and potential as a therapeutic target in cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MDMX Antibody (G-10) References:
- Targeting p53-MDM2-MDMX loop for cancer therapy. | Zhang, Q., et al. 2014. Subcell Biochem. 85: 281-319. PMID: 25201201
- The Roles of MDM2 and MDMX in Cancer. | Karni-Schmidt, O., et al. 2016. Annu Rev Pathol. 11: 617-44. PMID: 27022975
- Mouse modelling of the MDM2/MDMX-p53 signalling axis. | Tackmann, NR. and Zhang, Y. 2017. J Mol Cell Biol. 9: 34-44. PMID: 28096294
- MDM2 and MDMX promote ferroptosis by PPARα-mediated lipid remodeling. | Venkatesh, D., et al. 2020. Genes Dev. 34: 526-543. PMID: 32079652
- MDMX acts as a pervasive preleukemic-to-acute myeloid leukemia transition mechanism. | Ueda, K., et al. 2021. Cancer Cell. 39: 529-547.e7. PMID: 33667384
- MDMX drives pre-leukaemia progression. | Seton-Rogers, S. 2021. Nat Rev Cancer. 21: 280. PMID: 33762752
- The role of miRNAs in MDMX-p53 interplay. | Yu, D., et al. 2021. J Evid Based Med. 14: 152-160. PMID: 33988919
- Therapeutic opportunities in cancer therapy: targeting the p53-MDM2/MDMX interactions. | Munisamy, M., et al. 2021. Am J Cancer Res. 11: 5762-5781. PMID: 35018225
- The MDM2/MDMX/p53 axis in the adaptive stress response. | Wang, B., et al. 2020. Transl Cancer Res. 9: 1993-1997. PMID: 35117546
- Verbenalin attenuates hepatic damage and mitochondrial dysfunction in alcohol-associated steatohepatitis by regulating MDMX/PPARα-mediated ferroptosis. | Dong, J., et al. 2023. J Ethnopharmacol. 307: 116227. PMID: 36739928
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MDMX Antibody (G-10) | sc-74467 | 200 µg/ml | $322.00 | |||
MDMX Antibody (G-10): m-IgG Fc BP-HRP Bundle | sc-525716 | 200 µg Ab; 10 µg BP | $361.00 | |||
MDMX Antibody (G-10): m-IgG2a BP-HRP Bundle | sc-546376 | 200 µg Ab; 10 µg BP | $361.00 | |||
MDMX Antibody (G-10) AC | sc-74467 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MDMX Antibody (G-10) HRP | sc-74467 HRP | 200 µg/ml | $322.00 | |||
MDMX Antibody (G-10) FITC | sc-74467 FITC | 200 µg/ml | $336.00 | |||
MDMX Antibody (G-10) PE | sc-74467 PE | 200 µg/ml | $349.00 | |||
MDMX Antibody (G-10) Alexa Fluor® 488 | sc-74467 AF488 | 200 µg/ml | $364.00 | |||
MDMX Antibody (G-10) Alexa Fluor® 546 | sc-74467 AF546 | 200 µg/ml | $364.00 | |||
MDMX Antibody (G-10) Alexa Fluor® 594 | sc-74467 AF594 | 200 µg/ml | $364.00 | |||
MDMX Antibody (G-10) Alexa Fluor® 647 | sc-74467 AF647 | 200 µg/ml | $364.00 | |||
MDMX Antibody (G-10) Alexa Fluor® 680 | sc-74467 AF680 | 200 µg/ml | $364.00 | |||
MDMX Antibody (G-10) Alexa Fluor® 790 | sc-74467 AF790 | 200 µg/ml | $364.00 |