Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KLC1 Double Nickase Plasmid (h): sc-401881-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KLC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- KLC1 Double Nickase Plasmid (h) and KLC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KLC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KLC1 Antibody (L2): sc-58776
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KLC1 Double Nickase Plasmid (h) | sc-401881-NIC | 20 µg | $410.00 | |||
KLC1 Double Nickase Plasmid (h2) | sc-401881-NIC-2 | 20 µg | $410.00 |
KLC1 encodes kinesin light chain 1, an accessory subunit of kinesin-1 that links cargo adaptors to the KIF5 motor to drive ATP-dependent transport along microtubules. Through interactions with vesicles, mitochondria, endosomes, and RNA/protein complexes, KLC1 contributes to polarized trafficking required for neurite outgrowth, synaptic maintenance, and organelle distribution. KLC1-regulated axonal transport interfaces with cytoskeletal dynamics and intracellular signaling that coordinate neuronal homeostasis and stress responses. Altered kinesin-1 cargo handling and transport defects are frequently studied in the context of neurodegenerative processes and other disorders with disrupted intracellular trafficking.
KLC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KLC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KLC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KLC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KLC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.