IRF-4 Antibody (3E4): sc-130921
- IRF-4 Antibody (3E4) is a rat monoclonal IgG1, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 386-450 of IRF-4 of mouse origin
- Anti-IRF-4 Antibody (3E4) is recommended for detection of IRF-4 of mouse, rat and human origin by WB, IP, IF and FCM
- Anti-IRF-4 Antibody (3E4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IRF-4 Antibody (3E4). This work is in progress.
IRF-4 Antibody (3E4) is a rat monoclonal IgG1 antibody that detects IRF-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-IRF-4 antibody (3E4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Interferon regulatory factor-4 (IRF-4) is a crucial nuclear protein primarily found in lymphoid cells, where IRF-4 functions as a transcriptional activator. IRF-4 plays a significant role in the immune response by binding to the interferon-stimulated response element (ISRE) of the major histocompatibility complex (MHC) class I promoter, thereby regulating the expression of genes involved in immune signaling. IRF-4 is essential for the differentiation and function of various immune cell types, including T cells and B cells, making IRF-4 a vital component in the adaptive immune system. IRF-4 also interacts with other proteins such as PU.1, which is essential for the development of immune cells, highlighting IRF-4′s role in complex signaling pathways that govern immune responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IRF-4 Antibody (3E4) References:
- IFN-alpha and IL-12 activate IFN regulatory factor 1 (IRF-1), IRF-4, and IRF-8 gene expression in human NK and T cells. | Lehtonen, A., et al. 2003. Cytokine. 24: 81-90. PMID: 14581002
- E47, IRF-4, and PU.1 synergize to induce B-cell-specific activation of the class II transactivator promoter III (CIITA-PIII). | van der Stoep, N., et al. 2004. Blood. 104: 2849-57. PMID: 15242870
- Negative regulation of Toll-like-receptor signaling by IRF-4. | Negishi, H., et al. 2005. Proc Natl Acad Sci U S A. 102: 15989-94. PMID: 16236719
- E2A and IRF-4/Pip promote chromatin modification and transcription of the immunoglobulin kappa locus in pre-B cells. | Lazorchak, AS., et al. 2006. Mol Cell Biol. 26: 810-21. PMID: 16428437
- Loss of IRF-4-binding protein leads to the spontaneous development of systemic autoimmunity. | Fanzo, JC., et al. 2006. J Clin Invest. 116: 703-14. PMID: 16470246
- Studies of DNA recognition mechanism of transcription factor IRF-4. | Furuita, K., et al. 2006. Nucleic Acids Symp Ser (Oxf). 259-60. PMID: 17150916
- Immunohistochemical detection of multiple myeloma 1/interferon regulatory factor 4 (MUM1/IRF-4) in canine plasmacytoma: comparison with CD79a and CD20. | Ramos-Vara, JA., et al. 2007. Vet Pathol. 44: 875-84. PMID: 18039900
- Regulation of immunoglobulin light-chain recombination by the transcription factor IRF-4 and the attenuation of interleukin-7 signaling. | Johnson, K., et al. 2008. Immunity. 28: 335-45. PMID: 18280186
- PKC eta directs induction of IRF-4 expression and Ig kappa gene rearrangement in pre-BCR signaling pathway. | Oda, A., et al. 2008. Int Immunol. 20: 1417-26. PMID: 18780722
- IRF-4-binding protein inhibits interleukin-17 and interleukin-21 production by controlling the activity of IRF-4 transcription factor. | Chen, Q., et al. 2008. Immunity. 29: 899-911. PMID: 19062315
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRF-4 Antibody (3E4) | sc-130921 | 200 µg/ml | $322.00 | |||
IRF-4 Antibody (3E4) AC | sc-130921 AC | 500 µg/ml, 25% agarose | $424.00 | |||
IRF-4 Antibody (3E4) HRP | sc-130921 HRP | 200 µg/ml | $322.00 | |||
IRF-4 Antibody (3E4) FITC | sc-130921 FITC | 200 µg/ml | $336.00 | |||
IRF-4 Antibody (3E4) PE | sc-130921 PE | 200 µg/ml | $349.00 | |||
IRF-4 Antibody (3E4) Alexa Fluor® 488 | sc-130921 AF488 | 200 µg/ml | $364.00 | |||
IRF-4 Antibody (3E4) Alexa Fluor® 546 | sc-130921 AF546 | 200 µg/ml | $364.00 | |||
IRF-4 Antibody (3E4) Alexa Fluor® 594 | sc-130921 AF594 | 200 µg/ml | $364.00 | |||
IRF-4 Antibody (3E4) Alexa Fluor® 647 | sc-130921 AF647 | 200 µg/ml | $364.00 | |||
IRF-4 Antibody (3E4) Alexa Fluor® 680 | sc-130921 AF680 | 200 µg/ml | $364.00 | |||
IRF-4 Antibody (3E4) Alexa Fluor® 790 | sc-130921 AF790 | 200 µg/ml | $364.00 |