IP3KA Double Nickase Plasmid (h): sc-404638-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IP3KA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IP3KA Double Nickase Plasmid (h) and IP3KA Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITPKA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IP3KA Antibody (F-3): sc-271838
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IP3KA Double Nickase Plasmid (h) | sc-404638-NIC | 20 µg | $410.00 | |||
IP3KA Double Nickase Plasmid (h2) | sc-404638-NIC-2 | 20 µg | $410.00 |
Human ITPKA encodes inositol-trisphosphate 3-kinase A (IP3KA), a Ca2+/calmodulin-regulated enzyme that phosphorylates inositol 1,4,5-trisphosphate (IP3) to generate IP4, thereby shaping intracellular calcium signaling dynamics. By modulating IP3-dependent Ca2+ release from the endoplasmic reticulum, IP3KA influences synaptic function, cytoskeletal remodeling, and activity-dependent signaling programs, including pathways that couple calcium transients to transcriptional responses. In neurons, IP3KA is enriched in dendritic spines and has been linked to mechanisms underlying plasticity and excitability through regulation of Ca2+-sensitive effectors. Altered IP3/IP4 balance and calcium homeostasis associated with ITPKA dysregulation is relevant to neurobiology and has also been investigated in contexts of aberrant signaling and migration phenotypes in disease models.
IP3KA Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITPKA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITPKA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITPKA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITPKA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.