IGF2R/M6PR Double Nickase Plasmid (h): sc-401697-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IGF2R/M6PR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IGF2R/M6PR Double Nickase Plasmid (h) and IGF2R/M6PR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IGF2R. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IGF2R/M6PR Antibody (2G11): sc-53146
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGF2R/M6PR Double Nickase Plasmid (h) | sc-401697-NIC | 20 µg | $410.00 | |||
IGF2R/M6PR Double Nickase Plasmid (h2) | sc-401697-NIC-2 | 20 µg | $410.00 |
IGF2R (also known as IGF2R/M6PR) encodes the cation-independent mannose-6-phosphate receptor, a multifunctional trafficking protein that binds M6P-tagged lysosomal hydrolases and mediates their sorting from the trans-Golgi network and endosomes to lysosomes. It also functions as a scavenger receptor for extracellular IGF2, modulating growth factor availability and influencing signaling programs linked to cell proliferation and differentiation. Through its central role in endosomal–lysosomal transport, IGF2R impacts autophagy-lysosome function, receptor turnover, and proteostasis. Dysregulation of IGF2R has been associated with altered lysosomal homeostasis and growth factor signaling imbalances relevant to developmental phenotypes and cancer biology research.
IGF2R/M6PR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IGF2R locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IGF2R. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IGF2R function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IGF2R-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.