ICAD Antibody (D-6): sc-398431

ICAD Antibody (D-6) is a mouse monoclonal IgG1 antibody that detects ICAD in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ICAD, or Inhibitor of Caspase-Activated DNase, plays a crucial role in the regulation of apoptosis by forming a complex with CAD (Caspase-Activated DNase), thereby inhibiting CAD activity and preventing premature DNA fragmentation during the early stages of apoptosis. This interaction ensures DNA is only cleaved when the cell is fully committed to the apoptotic process, thus maintaining genomic integrity until the appropriate time. The cleavage of ICAD by caspases during apoptosis releases CAD, allowing CAD to translocate to the nucleus and induce DNA fragmentation, a hallmark of apoptosis. The precise regulation of this process is essential for normal cellular turnover and the prevention of diseases such as cancer, where dysregulation of apoptosis can lead to uncontrolled cell proliferation. Anti-ICAD antibody (D-6) serves as an invaluable tool for researchers studying the mechanisms of apoptosis and ICAD′s role in various biological contexts.

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ICAD Antibody (D-6) References:

1. The effect of ICAD-S on the formation and intracellular distribution of a nucleolytically active caspase-activated DNase.  |  Scholz, SR., et al. 2002. Nucleic Acids Res. 30: 3045-51. PMID: 12136086
2. Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes.  |  Todor, A., et al. 2002. Am J Physiol Heart Circ Physiol. 283: H990-5. PMID: 12181128
3. Decreased expression of DFF45/ICAD is correlated with a poor prognosis in patients with esophageal carcinoma.  |  Konishi, S., et al. 2002. Cancer. 95: 2473-8. PMID: 12467059
4. Expression, purification, crystallization of fragments from the C-terminal region of DFF45/ICAD.  |  Ding, H., et al. 2003. Acta Crystallogr D Biol Crystallogr. 59: 1323-6. PMID: 12832800
5. Evaluation of biofilm production and prevalence of the icaD gene in methicillin-resistant and methicillin-susceptible Staphylococcus aureus strains isolated from patients with nosocomial infections and carriers.  |  Grinholc, M., et al. 2007. FEMS Immunol Med Microbiol. 50: 375-9. PMID: 17537178
6. Caspase-2 cleaves DNA fragmentation factor (DFF45)/inhibitor of caspase-activated DNase (ICAD).  |  Dahal, GR., et al. 2007. Arch Biochem Biophys. 468: 134-9. PMID: 17945178
7. GCN5 and BCR signalling collaborate to induce pre-mature B cell apoptosis through depletion of ICAD and IAP2 and activation of caspase activities.  |  Kikuchi, H. and Nakayama, T. 2008. Gene. 419: 48-55. PMID: 18538956
8. Detection of icaA, icaD genes and biofilm production by Staphylococcus aureus and Staphylococcus epidermidis isolated from urinary tract catheterized patients.  |  Gad, GF., et al. 2009. J Infect Dev Ctries. 3: 342-51. PMID: 19759503
9. Loss of Pnn expression results in mouse early embryonic lethality and cellular apoptosis through SRSF1-mediated alternative expression of Bcl-xS and ICAD.  |  Leu, S., et al. 2012. J Cell Sci. 125: 3164-72. PMID: 22454513
10. Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses.  |  Kıvanç, SA., et al. 2017. Turk J Ophthalmol. 47: 68-73. PMID: 28405479

The preferred ICAD antibody is ICAD Antibody (F-8): sc-17816.