p-Histone H2A.X Antibody (Ser139-7G9-H4): sc-517558

p-Histone H2A.X Antibody (Ser139-7G9-H4) is a mouse monoclonal IgG antibody that detects p-Histone H2A.X in mouse, rat, and human samples through applications such as western blotting (WB) and immunofluorescence (IF). p-Histone H2A.X, also known as γ-H2A.X, is a phosphorylated variant of the histone H2A.X protein, which plays a crucial role in the cellular response to DNA damage, particularly in the recognition and repair of double-stranded breaks. Upon exposure to ionizing radiation or other genotoxic stress, H2A.X is rapidly phosphorylated at serine 139, leading to the formation of distinct nuclear foci that serve as recruitment sites for DNA repair proteins, including Rad50, Rad51, and BRCA1. This phosphorylation event is vital for initiating the DNA damage response, as γ-H2A.X marks the sites of damage and facilitates the assembly of repair complexes, thereby maintaining genomic stability. The presence of γ-H2A.X foci is not only a marker of DNA damage but also an indicator of the cell′s ability to respond to and repair such damage, making anti-p-Histone H2A.X antibody (Ser139-7G9-H4) an essential tool for researchers studying DNA repair mechanisms and the effects of genotoxic agents in various biological contexts.

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p-Histone H2A.X Antibody (Ser139-7G9-H4) References:

1. Effect of DNA damage response by quinazolinone analogue HMJ-38 on human umbilical vein endothelial cells: evidence for γH2A.X and DNA-PK-dependent pathway.  |  Chiang, JH., et al. 2014. Hum Exp Toxicol. 33: 590-601. PMID: 24064905
2. Non-toxic dose chidamide synergistically enhances platinum-induced DNA damage responses and apoptosis in Non-Small-Cell lung cancer cells.  |  Zhou, Y., et al. 2014. Biomed Pharmacother. 68: 483-91. PMID: 24721323
3. Inhibition of histone deacetylases enhances DNA damage repair in SCNT embryos.  |  Bohrer, RC., et al. 2014. Cell Cycle. 13: 2138-48. PMID: 24841373
4. Maternal diabetes triggers DNA damage and DNA damage response in neurulation stage embryos through oxidative stress.  |  Dong, D., et al. 2015. Biochem Biophys Res Commun. 467: 407-12. PMID: 26427872
5. Withaferin A Induces Oxidative Stress-Mediated Apoptosis and DNA Damage in Oral Cancer Cells.  |  Chang, HW., et al. 2017. Front Physiol. 8: 634. PMID: 28936177
6. Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation.  |  Gaudreau-Lapierre, A., et al. 2018. J Vis Exp.. PMID: 29630045
7. Evaluation of the cytotoxic and genotoxic potential of the captan-based fungicides, chlorothalonil-based fungicides and methyl thiophanate-based fungicides in human fibroblasts BJ.  |  Moo-Muñoz, AJ., et al. 2021. J Environ Sci Health B. 56: 877-883. PMID: 34486949
8. Synthesis of N-aryl-2,6-diphenyl-2H-pyrazolo[4,3-c]pyridin-7-amines and their photodynamic properties in the human skin melanoma cell line G361.  |  Razmienė, B., et al. 2022. Bioorg Chem. 119: 105570. PMID: 34953323
9. Organophosphorus Flame Retardant TCPP Induces Cellular Senescence in Normal Human Skin Keratinocytes: Implication for Skin Aging.  |  Liu, JX., et al. 2022. Int J Mol Sci. 23: PMID: 36430782
10. The influence of male HBV infection on sperm quality, embryonic development, and assisted reproductive outcomes.  |  Meng, X., et al. 2024. Hum Reprod. 39: 43-52. PMID: 37994690

The preferred Histone H2A.X antibody is Histone H2A.X Antibody (938CT5.1.1): sc-517336.