HEXB Double Nickase Plasmid (h): sc-404765-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HEXB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HEXB Double Nickase Plasmid (h) and HEXB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HEXB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HEXB B chain Antibody (D-9): sc-376781
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HEXB Double Nickase Plasmid (h) | sc-404765-NIC | 20 µg | $410.00 | |||
HEXB Double Nickase Plasmid (h2) | sc-404765-NIC-2 | 20 µg | $410.00 |
HEXB encodes the beta subunit of lysosomal β-hexosaminidase, which forms heterodimeric Hex A with HEXA and homodimeric Hex B to catalyze terminal N-acetylhexosamine removal from glycoconjugates. This activity is central to lysosomal glycosphingolipid and glycosaminoglycan turnover, supporting membrane lipid homeostasis and cellular recycling pathways. Disruption of HEXB impairs GM2 ganglioside degradation and related lysosomal catabolic processes, linking the gene to neurodegenerative lysosomal storage phenotypes. Accordingly, HEXB is widely used to study lysosome biology, lipid trafficking, and stress responses associated with substrate accumulation in human cells.
HEXB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HEXB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HEXB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HEXB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HEXB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.