Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HAP1 CRISPR/Cas9 KO Plasmid (h): sc-405778
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HAP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HAP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HAP1 Antibody (C-3): sc-166245
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAP1 CRISPR/Cas9 KO Plasmid (h) | sc-405778 | 20 µg | $397.00 |
Overview
HAP1 (huntingtin-associated protein 1) is a cytoplasmic scaffolding protein enriched in neurons that binds huntingtin and coordinates trafficking and signaling complexes. It contributes to microtubule-based transport, endosomal sorting, and vesicle dynamics, influencing receptor recycling and neurite outgrowth. HAP1 also interfaces with cytoskeletal regulators and motor proteins to modulate intracellular distribution of organelles and cargo. Altered HAP1-linked transport and proteostasis pathways are frequently studied in the context of neurodegenerative mechanisms, including Huntington’s disease-associated cellular stress phenotypes.
HAP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HAP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HAP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HAP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HAP1 protein expression.
This CRISPR knockout system enables efficient generation of HAP1-deficient cell models for investigation of HAP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HAP1 exon(s) critical for HAP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HAP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HAP1 CRISPR/Cas9 KO Plasmid (h) and HAP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HAP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HAP1 HDR Plasmid (h) and HAP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HAP1 homology arms to support homology-directed repair at defined HAP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.