GnRH I Antibody (C-9): sc-373690

GnRH I Antibody (C-9) is a mouse monoclonal IgG2a antibody that detects gonadotropin-releasing hormone (GnRH) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GnRH plays a crucial role in the regulation of human reproduction by orchestrating the hypothalamic-pituitary-gonadal axis, which is essential for the release of luteinizing hormone and follicle-stimulating hormone from the anterior pituitary. This decapeptide is produced by hypothalamic neurons and is secreted in a pulsatile manner, ensuring the proper timing of hormonal release necessary for reproductive function. GnRH is expressed in the hypothalamus, acrosomal region of human sperm, anterior pituitary, and certain cancer cells, indicating diverse roles beyond reproduction. A second form, GnRH II, primarily found in the midbrain and expressed in tissues such as the kidney, bone marrow, and prostate, suggests additional functions that are yet to be fully understood. GnRH binding to specific G protein-coupled receptors in the pituitary is a critical step in regulating the synthesis and secretion of gonadotropins, highlighting GnRH′s importance in both normal physiology and potential pathological conditions.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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GnRH I Antibody (C-9) References:

1. The neuropeptides GnRH-II and GnRH-I are produced by human T cells and trigger laminin receptor gene expression, adhesion, chemotaxis and homing to specific organs.  |  Chen, A., et al. 2002. Nat Med. 8: 1421-6. PMID: 12447356
2. Molecular biology of gonadotropin-releasing hormone (GnRH)-I, GnRH-II, and their receptors in humans.  |  Cheng, CK. and Leung, PC. 2005. Endocr Rev. 26: 283-306. PMID: 15561800
3. Analogs of GnRH-I and GnRH-II inhibit epidermal growth factor-induced signal transduction and resensitize resistant human breast cancer cells to 4OH-tamoxifen.  |  Günthert, AR., et al. 2005. Eur J Endocrinol. 153: 613-25. PMID: 16189183
4. Human neurons express type I GnRH receptor and respond to GnRH I by increasing luteinizing hormone expression.  |  Wilson, AC., et al. 2006. J Endocrinol. 191: 651-63. PMID: 17170222
5. GnRH-I and GnRH-II-induced calcium signaling and hormone secretion in neonatal rat gonadotrophs.  |  Balík, A., et al. 2009. Physiol Res. 58: 709-716. PMID: 19093727
6. Identification of European starling GnRH-I precursor mRNA and its seasonal regulation.  |  Ubuka, T., et al. 2009. Gen Comp Endocrinol. 162: 301-6. PMID: 19362556
7. Gonadotropin-releasing hormone type II (GnRH-II) agonist regulates the invasiveness of endometrial cancer cells through the GnRH-I receptor and mitogen-activated protein kinase (MAPK)-dependent activation of matrix metalloproteinase (MMP)-2.  |  Wu, HM., et al. 2013. BMC Cancer. 13: 300. PMID: 23786715
8. Synthesis, characterization and systematic comparison of FITC-labelled GnRH-I, -II and -III analogues on various tumour cells.  |  Murányi, J., et al. 2016. J Pept Sci. 22: 552-60. PMID: 27443981
9. Simulating Peptide Monolayer Formation: GnRH-I on Silica.  |  Pujol-Navarro, N., et al. 2021. Int J Mol Sci. 22: PMID: 34073815
10. Lactate-Dependent Cross-Talk Between Astrocyte and GnRH-I Neurons in Hypothalamus of Aged Brain: Decreased GnRH-I Transcription.  |  Das, M., et al. 2022. Reprod Sci. 29: 2546-2564. PMID: 35138586

The preferred GnRH I antibody is GnRH I Antibody (HU11B): sc-32292.