Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
gamma Synuclein/SNCG CRISPR/Cas9 KO Plasmid (h): sc-401050
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- gamma Synuclein/SNCG CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the gamma Synuclein/SNCG genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: gamma Synuclein/SNCG Antibody (1H10D2): sc-65979
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
gamma Synuclein/SNCG CRISPR/Cas9 KO Plasmid (h) | sc-401050 | 20 µg | $397.00 |
Overview
SNCG encodes gamma-synuclein, a small presynaptic-associated protein implicated in modulation of cytoskeletal organization, vesicle trafficking, and cellular stress responses. In non-neuronal contexts, SNCG expression has been linked to altered cell motility and signaling through pathways that couple microtubule dynamics with membrane transport and proteostasis. Dysregulated gamma-synuclein has been reported in multiple tumor types and is frequently studied for its associations with invasion, metastasis-related phenotypes, and resistance to cellular stress. As a member of the synuclein family, SNCG also provides a comparative model for investigating protein aggregation biology and synaptic-like vesicle processes in diverse cell systems.
gamma Synuclein/SNCG CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SNCG gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SNCG together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SNCG open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish gamma Synuclein/SNCG protein expression.
This CRISPR knockout system enables efficient generation of SNCG-deficient cell models for investigation of gamma Synuclein/SNCG signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SNCG exon(s) critical for gamma Synuclein/SNCG function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SNCG genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by gamma Synuclein/SNCG CRISPR/Cas9 KO Plasmid (h) and gamma Synuclein/SNCG CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SNCG locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by gamma Synuclein/SNCG HDR Plasmid (h) and gamma Synuclein/SNCG HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SNCG homology arms to support homology-directed repair at defined SNCG target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.