GADD 45 alpha Antibody (A-3): sc-515568
- GADD 45α Antibody (A-3) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 140-165 at the C-terminus of GADD 45α of human origin
- recommended for detection of GADD 45α of mouse, rat and human origin by WB, IP, IF and ELISA
- See GADD 45α (C-4): sc-6850 for GADD 45α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GADD 45α Antibody (A-3) for WB applications. These reagents are now offered in bundles with GADD 45α Antibody (A-3) (see ordering information below).
GADD 45α Antibody (A-3) is a mouse monoclonal IgG2a antibody that detects GADD 45α in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GADD 45α plays a crucial role in the cellular response to DNA damage, acting as a mediator between the p53 tumor suppressor pathway and DNA repair mechanisms. GADD 45α interacts with cyclin-dependent kinases (Cdks) and proliferating cell nuclear antigen (PCNA), which are essential for cell cycle regulation and DNA replication. Through binding to these proteins, GADD 45α inhibits Cdk activity, preventing cell cycle progression while enhancing DNA excision repair processes. This dual functionality underscores GADD 45α′s importance in maintaining genomic stability and preventing tumorigenesis, making GADD 45α a vital target for research in cancer biology and therapeutic development. Anti-GADD 45α antibody (A-3) serves as an invaluable tool for scientists investigating cell cycle regulation and DNA repair pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GADD 45 alpha Antibody (A-3) References:
- Nucleolin links to arsenic-induced stabilization of GADD45alpha mRNA. | Zhang, Y., et al. 2006. Nucleic Acids Res. 34: 485-95. PMID: 16421274
- Gadd45alpha regulates p38-dependent dendritic cell cytokine production and Th1 differentiation. | Jirmanova, L., et al. 2007. J Immunol. 178: 4153-8. PMID: 17371971
- GADD45α and annexin A1 are involved in the apoptosis of HL-60 induced by resveratrol. | Li, G., et al. 2011. Phytomedicine. 18: 704-9. PMID: 21277758
- JunD-mediated repression of GADD45α and γ regulates escape from cell death in prostate cancer. | Zerbini, LF., et al. 2011. Cell Cycle. 10: 2583-91. PMID: 21734453
- GADD45α inhibition of DNMT1 dependent DNA methylation during homology directed DNA repair. | Lee, B., et al. 2012. Nucleic Acids Res. 40: 2481-93. PMID: 22135303
- Abnormal levels of Gadd45alpha in developing neocortex impair neurite outgrowth. | Sarkisian, MR. and Siebzehnrubl, D. 2012. PLoS One. 7: e44207. PMID: 22970179
- GADD45α induction by nickel negatively regulates JNKs/p38 activation via promoting PP2Cα expression. | Yu, Y., et al. 2013. PLoS One. 8: e57185. PMID: 23536762
- HDAC inhibitors induce apoptosis but not cellular senescence in Gadd45α-deficient E1A+Ras cells. | Igotti Abramova, MV., et al. 2014. Int J Biochem Cell Biol. 51: 102-10. PMID: 24721210
- Progesterone suppresses the progression of colonic carcinoma by increasing the activity of the GADD45α/JNK/c‑Jun signalling pathway. | Zhang, YL., et al. 2021. Oncol Rep. 45: PMID: 33846816
- Correction: Flavagline analog FL3 induces cell cycle arrest in urothelial carcinoma cell of the bladder by inhibiting the Akt/PHB interaction to activate the GADD45α pathway. | Yuan, G., et al. 2022. J Exp Clin Cancer Res. 41: 291. PMID: 36180869
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GADD 45α Antibody (A-3) | sc-515568 | 200 µg/ml | $322.00 | |||
GADD 45α Antibody (A-3): m-IgG Fc BP-HRP Bundle | sc-538804 | 200 µg Ab; 10 µg BP | $361.00 | |||
GADD 45α Antibody (A-3): m-IgGκ BP-HRP Bundle | sc-536477 | 200 µg Ab; 40 µg BP | $361.00 | |||
GADD 45α Antibody (A-3): m-IgG2a BP-HRP Bundle | sc-548414 | 200 µg Ab; 10 µg BP | $361.00 | |||
GADD 45α (A-3) Neutralizing Peptide | sc-515568 P | 100 µg/0.5 ml | $69.00 |