eRF1 Antibody (E-11): sc-365653
- eRF1 Antibody (E-11) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 411-437 at the C-terminus of eRF1 of human origin
- recommended for detection of eRF1 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
- m-IgGκ BP-HRP is the preferred secondary detection reagent for eRF1 Antibody (E-11) for WB applications. This reagent is now offered in a bundle with eRF1 Antibody (E-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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eRF1 Antibody (E-11) is a mouse monoclonal IgG3 antibody that detects eRF1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eRF1, or eukaryotic release factor 1, plays a crucial role in the termination of protein synthesis, a vital process for cellular function and homeostasis. eRF1 is located in the cytoplasm, where eRF1 interacts with the ribosome to facilitate the release of newly synthesized polypeptides upon encountering stop codons. The proper functioning of eRF1 is essential, as any disruption in this process can lead to the accumulation of incomplete proteins, potentially resulting in cellular stress or disease. Notably, eRF1 forms a quaternary complex with the GTP-binding protein eRF3, which enhances activity and ensures accurate termination of translation. The gene encoding human eRF1 is located on chromosome 5q31.2, and eRF1′s highly conserved structure, including the essential Asn-Ile-Lys-Ser (NIKS) tetrapeptide, underscores importance in maintaining the fidelity of protein synthesis across different species. With broad reactivity and versatile applications, anti-eRF1 antibody (E-11) is an invaluable tool for researchers studying the intricacies of translation termination and implications in various biological processes.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eRF1 Antibody (E-11) | sc-365653 | 200 µg/ml | $322.00 | |||
eRF1 Antibody (E-11): m-IgGκ BP-HRP Bundle | sc-522314 | 200 µg Ab, 40 µg BP | $361.00 | |||
eRF1 (E-11) Neutralizing Peptide | sc-365653 P | 100 µg/0.5 ml | $69.00 |