Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ERCC1 Double Nickase Plasmid (h): sc-400630-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ERCC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ERCC1 Double Nickase Plasmid (h) and ERCC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ERCC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ERCC1 Antibody (D-10): sc-17809
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERCC1 Double Nickase Plasmid (h) | sc-400630-NIC | 20 µg | $410.00 | |||
ERCC1 Double Nickase Plasmid (h2) | sc-400630-NIC-2 | 20 µg | $410.00 |
ERCC1 encodes a structure-specific endonuclease that forms a heterodimer with XPF (ERCC4) to execute 5′ incision during nucleotide excision repair and to process DNA interstrand crosslinks and stalled replication intermediates. This complex functions in genome maintenance pathways that resolve helix-distorting lesions and coordinate repair with replication, thereby limiting accumulation of DNA damage and chromosomal aberrations. ERCC1 activity is tightly linked to cellular responses to UV-induced photoproducts and a range of bulky adducts, with downstream impacts on cell-cycle progression and apoptosis signaling. Dysregulated ERCC1-mediated repair capacity has been associated with altered genomic stability phenotypes and is frequently interrogated in studies of DNA damage response networks and mutational processes.
ERCC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ERCC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ERCC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ERCC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ERCC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.