eIF4E Antibody (A-10): sc-271480

eIF4E Antibody (A-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects eIF4E protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-eIF4E antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF4E protein plays a crucial role in the initiation of protein synthesis in eukaryotic cells, primarily by binding to the 5′ cap of mRNA, which is essential for the recruitment of the ribosome to the mRNA. This interaction facilitates the formation of the eukaryotic initiation complex eIF4F, which consists of eIF4E, eIF4A, and eIF4G. The complex promotes ribosome binding and helps in unwinding mRNA secondary structures, thereby enhancing translation efficiency. eIF4G serves as a scaffold that links eIF4E and eIF4A to the eIF3 complex, further underscoring eIF4E′s importance in regulating protein synthesis and cellular function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

eIF4E Antibody (A-10) References:

1. The emerging roles of translation factor eIF4E in the nucleus.  |  Strudwick, S. and Borden, KL. 2002. Differentiation. 70: 10-22. PMID: 11963652
2. eIF4E--from translation to transformation.  |  Mamane, Y., et al. 2004. Oncogene. 23: 3172-9. PMID: 15094766
3. eIF4E, the mRNA cap-binding protein: from basic discovery to translational research.  |  Sonenberg, N. 2008. Biochem Cell Biol. 86: 178-83. PMID: 18443631
4. Structure and functions of the translation initiation factor eIF4E and its role in cancer development and treatment.  |  Piserà, A., et al. 2018. J Genet Genomics. 45: 13-24. PMID: 29396141
5. Biochemical and Structural Insights into the Eukaryotic Translation Initiation Factor eIF4E.  |  Volpon, L., et al. 2019. Curr Protein Pept Sci. 20: 525-535. PMID: 30636602
6. Monitoring eIF4E-Dependent Nuclear 3' End mRNA Cleavage.  |  Delaleau, M. 2021. Methods Mol Biol. 2209: 347-361. PMID: 33201480
7. The versatile relationships between eIF4E and eIF4E-interacting proteins.  |  Hernández, G. 2022. Trends Genet. 38: 801-804. PMID: 35568601
8. miR-34b-3p Inhibition of eIF4E Causes Post-stroke Depression in Adult Mice.  |  Ke, X., et al. 2023. Neurosci Bull. 39: 194-212. PMID: 35802246
9. The eukaryotic translation initiation factor eIF4E reprograms alternative splicing.  |  Ghram, M., et al. 2023. EMBO J. 42: e110496. PMID: 36843541
10. Phospho-eIF4E stimulation regulates coronavirus entry by selective expression of cell membrane-residential factors.  |  Zhang, X-Z., et al. 2024. J Virol. 98: e0194823. PMID: 38299843

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF4E Antibody (A-10) has 37 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF4E Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF4E Antibody (A-10) Specifications

Alternate Product Name: eIF4E

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-217

Epitope Target: eIF4E

Epitope Species: human

Product Reactivity/Detection: eIF4E

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 37 citations in various scientific publications

Available Conjugates/Forms for eIF4E Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred eIF4E antibody is eIF4E Antibody (P-2): sc-9976.