Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DR5 Double Nickase Plasmid (h): sc-401002-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DR5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DR5 Double Nickase Plasmid (h) and DR5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TNFRSF10B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DR5 Antibody (D-6): sc-166624
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DR5 Double Nickase Plasmid (h) | sc-401002-NIC | 20 µg | $410.00 | |||
DR5 Double Nickase Plasmid (h2) | sc-401002-NIC-2 | 20 µg | $410.00 |
TNFRSF10B encodes death receptor 5 (DR5), a cell-surface member of the TNF receptor superfamily that transduces apoptotic signaling upon engagement by TRAIL. Receptor activation promotes DISC assembly with FADD and initiator caspases, linking extrinsic apoptosis to mitochondrial amplification and broader stress-response programs. DR5 signaling interfaces with NF-κB and MAPK pathways, shaping inflammatory and survival outputs in a context-dependent manner. Altered DR5 expression or pathway coupling is frequently studied in cancer and immune biology as a determinant of apoptosis sensitivity, cell fate decisions, and tumor–microenvironment interactions.
DR5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TNFRSF10B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TNFRSF10B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TNFRSF10B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TNFRSF10B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.