Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dnmt3a Double Nickase Plasmid (h): sc-400323-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dnmt3a Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dnmt3a Double Nickase Plasmid (h) and Dnmt3a Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DNMT3A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dnmt3a Antibody (C-12): sc-365769
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dnmt3a Double Nickase Plasmid (h) | sc-400323-NIC | 20 µg | $410.00 | |||
Dnmt3a Double Nickase Plasmid (h2) | sc-400323-NIC-2 | 20 µg | $410.00 |
DNMT3A encodes Dnmt3a, a de novo DNA methyltransferase that establishes CpG methylation patterns during development and cell fate commitment, shaping chromatin accessibility and long-term transcriptional programs. Dnmt3a cooperates with DNMT3B for methylation establishment and with DNMT1 for maintenance during DNA replication, integrating with epigenetic remodeling pathways that influence genome stability and transposon silencing. Altered DNMT3A function is linked to dysregulated hematopoietic differentiation and broad epigenomic reprogramming, and recurrent somatic variants are frequently studied in myeloid malignancy models. As a core regulator of methylation-dependent gene control, DNMT3A is commonly interrogated in stem cell biology, immune cell maturation, and cancer epigenetics research.
Dnmt3a Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DNMT3A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DNMT3A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DNMT3A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DNMT3A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.