Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CPS1 Double Nickase Plasmid (h): sc-402014-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CPS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CPS1 Double Nickase Plasmid (h) and CPS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CPS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CPS1 Antibody (B-1): sc-376190
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPS1 Double Nickase Plasmid (h) | sc-402014-NIC | 20 µg | $410.00 | |||
CPS1 Double Nickase Plasmid (h2) | sc-402014-NIC-2 | 20 µg | $410.00 |
Carbamoyl-phosphate synthase 1 (CPS1) encodes the mitochondrial enzyme that catalyzes the rate-limiting step of the urea cycle, converting ammonia and bicarbonate into carbamoyl phosphate in an ATP-dependent reaction. By controlling nitrogen disposal and arginine biosynthesis, CPS1 links mitochondrial metabolism to hepatocellular detoxification processes and broader amino acid and nucleotide metabolic networks. Altered CPS1 activity is associated with hyperammonemia and urea cycle disorders, and its expression can be remodeled in metabolic disease and cancer to support changes in nitrogen handling. These properties make CPS1 a relevant target for dissecting mitochondrial nitrogen flux, urea cycle regulation, and metabolic rewiring in human cell models.
CPS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CPS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CPS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CPS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CPS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.