Coronin 1A CRISPR Activation Plasmid (h): sc-403011-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Coronin 1A CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Coronin 1A CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Coronin 1A CRISPR Activation Plasmid (h) and Coronin 1A CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the CORO1A transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Coronin 1A Antibody (14.1): sc-100925
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Coronin 1A CRISPR Activation Plasmid (h) | sc-403011-ACT | 20 µg | $397.00 |
Human CORO1A encodes coronin 1A, an actin-binding WD-repeat protein enriched in hematopoietic cells that regulates cytoskeletal remodeling, cell migration, and immune synapse dynamics. Coronin 1A coordinates actin-dependent signaling events downstream of antigen receptors and chemokine cues, influencing vesicle trafficking, calcium flux, and survival pathways in leukocytes. Perturbation of CORO1A expression or function has been associated with immune dysregulation, including defects in T-cell homeostasis and susceptibility to infection, making it relevant for studying inflammatory and immunodeficiency-associated mechanisms. As a cytoskeleton-linked regulator, CORO1A is also used as a node to connect actin dynamics with signaling networks controlling lymphocyte activation and motility.
Coronin 1A CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous CORO1A expression without altering the underlying DNA sequence.
Coronin 1A CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the CORO1A locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the CORO1A transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Coronin 1A expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native CORO1A locus and enabling the study of Coronin 1A-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Coronin 1A pathway restoration in tumor cells with silenced or reduced CORO1A expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.